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KD-Validated Anti-Smac/Diablo Rabbit Monoclonal Antibody #61458

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Flow cytometric analysis of Smac/Diabl expression in HepG2 cells using Smac/Diabl antibody (Cat#61458, 1:2,000). Green, isotype control; red, Smac/Diabl.
Immunocytochemical staining of HepG2 cells with anti-Smac/Diablo antibody (Cat#61458, 1:1,000). Nuclei were stained blue with DAPI; Smac/Diablo was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-Smac/Diablo antibody (Cat#61458). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Smac/Diablo antibody (Cat#61458, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-Smac/Diablo antibody (Cat#61458). Smac/Diablo expression in wild-type (WT) and DIABLO knockdown (KD) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Smac/Diablo antibody (Cat#61458, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • 基因名：
    DIABLO
  • 货号：
    61458
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information
Product Name KD-Validated Anti-Smac/Diablo Rabbit Monoclonal Antibody
Aliases DIABLO; Diablo IAP-Binding Mitochondrial Protein; SMAC; Second Mitochondria-Derived Activator Of Caspase; DFNA64; Direct IAP-Binding Protein With Low PI; Diablo Homolog, Mitochondrial; DIABLO-S; FLJ25049; FLJ10537; Diablo, IAP-Binding Mitochondrial Protein; Diablo-Like Protein; Smac
Background

Gene Name: DIABLO

NCBI Gene Entry: 56616

UniProt Entry: Q9NR28
Application Information

Molecular Weight: Predicted, 27 kDa; observed, 21 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB2575

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human Smac/Diablo
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of Smac/Diabl expression in HepG2 cells using Smac/Diabl antibody (Cat#61458, 1:2,000). Green, isotype control; red, Smac/Diabl.
  • Immunocytochemical staining of HepG2 cells with anti-Smac/Diablo antibody (Cat#61458, 1:1,000). Nuclei were stained blue with DAPI; Smac/Diablo was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
  • Western blotting analysis using anti-Smac/Diablo antibody (Cat#61458). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Smac/Diablo antibody (Cat#61458, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using anti-Smac/Diablo antibody (Cat#61458). Smac/Diablo expression in wild-type (WT) and DIABLO knockdown (KD) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Smac/Diablo antibody (Cat#61458, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Citation(0)
  • 基因名：
    DIABLO
  • 货号：
    61458
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00
RELATED PRODUCTS
Flow cytometric analysis of Smac/Diabl expression in HepG2 cells using Smac/Diabl antibody (Cat#61458, 1:2,000). Green, isotype control; red, Smac/Diabl.
Immunocytochemical staining of HepG2 cells with anti-Smac/Diablo antibody (Cat#61458, 1:1,000). Nuclei were stained blue with DAPI; Smac/Diablo was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-Smac/Diablo antibody (Cat#61458). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Smac/Diablo antibody (Cat#61458, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-Smac/Diablo antibody (Cat#61458). Smac/Diablo expression in wild-type (WT) and DIABLO knockdown (KD) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Smac/Diablo antibody (Cat#61458, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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