Flow cytometric analysis of Wnt family member 5A expression in HepG2 cells using Wnt family member 5A antibody (Cat#61523, 1:2,000). Green, isotype control; red, Wnt family member 5A.
Immunocytochemical staining of HepG2 cells with Wnt family member 5A antibody (Cat#61523, 1:1,000). Nuclei were stained blue with DAPI; Wnt family member 5A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
Western blotting analysis using anti-Wnt family member 5A antibody (Cat#61523). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Wnt family member 5A antibody (Cat#61523, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-Wnt family member 5A antibody (Cat#61523). Wnt family member 5A expression in wild type (WT) and Wnt family member 5A shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Wnt family member 5A antibody (Cat#61523, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
