Flow cytometric analysis of vimentin expression in C2C12 cells using anti-vimentin antibody (Cat#61538, 1:2,000). Green, isotype control; red, vimentin.
Immunocytochemical staining of C2C12 cells with anti-Vimentin antibody (Cat#61538, 1:1,000). Nuclei were stained blue with DAPI; Vimentin was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Western blotting analysis using anti-vimentin antibody (Cat#61538). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-vimentin antibody (Cat#61538, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-vimentin antibody (Cat#61538). Vimentin expression in wild type (WT) and vimentin (VIM) knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-vimentin antibody (Cat#61538, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Immunohistochemistry was performed on paraffin-embedded human endometrial carcinoma using anti-vimentin antibody (Cat#61538, 1:100). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
