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KD-Validated Anti-AIFM1 Rabbit Monoclonal Antibody #61553

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Flow cytometric analysis of AIFM1 expression in HepG2 cells using anti-AIFM1 antibody (Cat#61553, 1:2,000). Green, isotype control; red, AIFM1.
Immunocytochemical staining of HepG2 cells with anti-AIFM1 antibody (Cat#61553, 1:1,000). Nuclei were stained blue with DAPI; AIFM1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Western blotting analysis using anti-AIFM1 antibody (Cat#61553). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-AIFM1 antibody (Cat#61553, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-AIFM1 antibody (Cat#61553). AIFM1 expression in wild-type (WT) and AIFM1 knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-AIFM1 antibody (Cat#61553, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • 基因名：
    AIFM1
  • 货号：
    61553
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information
Product Name KD-Validated Anti-AIFM1 Rabbit Monoclonal Antibody
Aliases AIFM1; Apoptosis Inducing Factor Mitochondria Associated 1; AIF; CMTX4; DFNX5; PDCD8; Apoptosis-Inducing Factor, Mitochondrion-Associated, 1; Programmed Cell Death 8 (Apoptosis-Inducing Factor); Apoptosis-Inducing Factor 1, Mitochondrial; Auditory Neuropathy, X-Linked Recessive 1; AUNX1; NAMSD; Neuropathy,Axonal, Motor-Sensory With Deafness And Mental Retardation (Cowchock Syndrome); Apoptosis Inducing Factor, Mitochondria Associated 1; Striatal Apoptosis-Inducing Factor; Testicular Secretory Protein Li 4; Programmed Cell Death Protein 8; EC 1.6.99.-; COXPD6; SEMDHL; CMT2D; COWCK; NADMR
Background

Gene Name: AIFM1

NCBI Gene Entry: 9131

UniProt Entry: O95831
Application Information

Molecular Weight: Predicted, 67 kDa, observed, 67 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB2810

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human AIF
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of AIFM1 expression in HepG2 cells using anti-AIFM1 antibody (Cat#61553, 1:2,000). Green, isotype control; red, AIFM1.
  • Immunocytochemical staining of HepG2 cells with anti-AIFM1 antibody (Cat#61553, 1:1,000). Nuclei were stained blue with DAPI; AIFM1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
  • Western blotting analysis using anti-AIFM1 antibody (Cat#61553). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-AIFM1 antibody (Cat#61553, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using anti-AIFM1 antibody (Cat#61553). AIFM1 expression in wild-type (WT) and AIFM1 knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-AIFM1 antibody (Cat#61553, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Citation(0)
  • 基因名：
    AIFM1
  • 货号：
    61553
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00
RELATED PRODUCTS
Flow cytometric analysis of AIFM1 expression in HepG2 cells using anti-AIFM1 antibody (Cat#61553, 1:2,000). Green, isotype control; red, AIFM1.
Immunocytochemical staining of HepG2 cells with anti-AIFM1 antibody (Cat#61553, 1:1,000). Nuclei were stained blue with DAPI; AIFM1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Western blotting analysis using anti-AIFM1 antibody (Cat#61553). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-AIFM1 antibody (Cat#61553, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-AIFM1 antibody (Cat#61553). AIFM1 expression in wild-type (WT) and AIFM1 knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-AIFM1 antibody (Cat#61553, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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