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KD-Validated Anti-Acetyl-CoA acyltransfe rase 2 Rabbit Monoclonal Antibody #61563

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Flow cytometric analysis of Acetyl-CoA acyltransferase 2 expression in C2C12 cells using Acetyl-CoA acyltransferase 2 antibody (Cat#61563, 1:2,000). Green, isotype control; red, Acetyl-CoA acyltransferase 2.
Immunocytochemical staining of C2C12 cells with Acetyl-CoA acyltransferase 2 antibody (Cat#61563, 1:1,000). Nuclei were stained blue with DAPI; Acetyl-CoA acyltransferase 2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
Western blotting analysis using anti-Acetyl-CoA acyltransferase 2 antibody (Cat#61563). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Acetyl-CoA acyltransferase 2 antibody (Cat#61563, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-Acetyl-CoA acyltransferase 2 antibody (Cat#61563). Acetyl-CoA acyltransferase 2 expression in wild type (WT) and Acetyl-CoA acyltransferase 2 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Acetyl-CoA acyltransferase 2 antibody (Cat#61563, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Validation of Acetyl-CoA acyltransferase 2 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-Acetyl-CoA acyltransferase 2 antibody (Cat#61563, 1:2,000) and analyzed using BD flow cytometer.
Immunocytochemical staining of HeLa cells using anti-Acetyl-CoA acyltransferase 2 antibody (Cat#61563, 1:1,000), Top panel: wild-type (WT); Bottom panal: Acetyl-CoA acyltransferase 2 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Acetyl-CoA acyltransferase 2 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.
  • 基因名：
    ACAA2
  • 货号：
    61563
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information
Product Name KD-Validated Anti-Acetyl-CoA acyltransfe rase 2 Rabbit Monoclonal Antibody
Aliases ACAA2; Acetyl-CoA Acyltransferase 2; DSAEC 2; Mitochondrial 3-Oxoacyl-Coenzyme A Thiolase 2; 3-Ketoacyl-CoA Thiolase, Mitochondrial; Mitochondrial 3-Oxoacyl-CoA Thiolase; Acetyl-Coenzyme A Acyltransferase 2; Acyl-CoA Hydrolase, Mitochondrial; Acetyl-CoA Acetyltransferase; EC 2.3.1.16; T1; Acetyl-CoA Acyltransferase; Beta Ketothiolase; Beta-Ketothiolase; EC 2.3.1.9; EC 3.1.2.-; EC 3.1.2.1; EC 3.1.2.2; EC 2.3.1
Background

Gene Name: ACAA2

NCBI Gene Entry: 10449

UniProt Entry: P42765
Application Information

Molecular Weight: Predicted, 42 kDa, observed, 45 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB2955

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human ACAA2
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of Acetyl-CoA acyltransferase 2 expression in C2C12 cells using Acetyl-CoA acyltransferase 2 antibody (Cat#61563, 1:2,000). Green, isotype control; red, Acetyl-CoA acyltransferase 2.
  • Immunocytochemical staining of C2C12 cells with Acetyl-CoA acyltransferase 2 antibody (Cat#61563, 1:1,000). Nuclei were stained blue with DAPI; Acetyl-CoA acyltransferase 2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
  • Western blotting analysis using anti-Acetyl-CoA acyltransferase 2 antibody (Cat#61563). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Acetyl-CoA acyltransferase 2 antibody (Cat#61563, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using anti-Acetyl-CoA acyltransferase 2 antibody (Cat#61563). Acetyl-CoA acyltransferase 2 expression in wild type (WT) and Acetyl-CoA acyltransferase 2 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Acetyl-CoA acyltransferase 2 antibody (Cat#61563, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Validation of Acetyl-CoA acyltransferase 2 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-Acetyl-CoA acyltransferase 2 antibody (Cat#61563, 1:2,000) and analyzed using BD flow cytometer.
  • Immunocytochemical staining of HeLa cells using anti-Acetyl-CoA acyltransferase 2 antibody (Cat#61563, 1:1,000), Top panel: wild-type (WT); Bottom panal: Acetyl-CoA acyltransferase 2 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Acetyl-CoA acyltransferase 2 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.
Citation(0)
  • 基因名：
    ACAA2
  • 货号：
    61563
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00
RELATED PRODUCTS
Flow cytometric analysis of Acetyl-CoA acyltransferase 2 expression in C2C12 cells using Acetyl-CoA acyltransferase 2 antibody (Cat#61563, 1:2,000). Green, isotype control; red, Acetyl-CoA acyltransferase 2.
Immunocytochemical staining of C2C12 cells with Acetyl-CoA acyltransferase 2 antibody (Cat#61563, 1:1,000). Nuclei were stained blue with DAPI; Acetyl-CoA acyltransferase 2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
Western blotting analysis using anti-Acetyl-CoA acyltransferase 2 antibody (Cat#61563). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Acetyl-CoA acyltransferase 2 antibody (Cat#61563, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-Acetyl-CoA acyltransferase 2 antibody (Cat#61563). Acetyl-CoA acyltransferase 2 expression in wild type (WT) and Acetyl-CoA acyltransferase 2 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Acetyl-CoA acyltransferase 2 antibody (Cat#61563, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Validation of Acetyl-CoA acyltransferase 2 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-Acetyl-CoA acyltransferase 2 antibody (Cat#61563, 1:2,000) and analyzed using BD flow cytometer.
Immunocytochemical staining of HeLa cells using anti-Acetyl-CoA acyltransferase 2 antibody (Cat#61563, 1:1,000), Top panel: wild-type (WT); Bottom panal: Acetyl-CoA acyltransferase 2 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Acetyl-CoA acyltransferase 2 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.
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