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KD-Validated Anti-ARID1A Rabbit Monoclonal Antibody #61580

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Flow cytometric analysis of ARID1A expression in C2C12 cells using ARID1A antibody (Cat#61580, 1:2000). Green, isotype control; red, ARID1A.
Immunocytochemical staining of C2C12 cells with ARID1A antibody (Cat#61580, 1:1,000). Nuclei were stained blue with DAPI; ARID1A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-ARID1A antibody (Cat#61580). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ARID1A antibody (Cat#61580, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-ARID1A antibody (Cat#61580). ARID1A expression in wild type (WT) and ARID1A shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-ARID1A antibody (Cat#61580, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Validation of ARID1A knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-ARID1A antibody (Cat#61580, 1:2,000) and analyzed using BD flow cytometer.
Immunocytochemical staining of Hela cells using anti-ARID1A antibody (Cat#61580, 1:1,000), Top panel: wild-type (WT); Bottom panal: ARID1A shRNA knockdown (KD). Nuclei were stained blue with DAPI; ARID1A was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
  • 基因名：
    ARID1A
  • 货号：
    61580
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information
Product Name KD-Validated Anti-ARID1A Rabbit Monoclonal Antibody
Aliases ARID1A; AT-Rich Interaction Domain 1A; BAF250; B120; SMARCF1; P270; SWI/SNF-Related, Matrix-Associated, Actin-Dependent Regulator Of Chromatin Subfamily F Member 1; AT-Rich Interactive Domain-Containing Protein 1A; AT Rich Interactive Domain 1A (SWI-Like); ARID Domain-Containing Protein 1A; SWI/SNF Complex Protein P270; BRG1-Associated Factor 250a; SWI-Like Protein; Osa Homolog 1; BAF250a; BAF250A; C1orf4; HOSA1; OSA1; HELD; SWI/SNF Related, Matrix Associated, Actin Dependent Regulator Of Chromatin, Subfamily F, Member 1; AT Rich Interactive Domain 1A (SWI- Like); Chromatin Remodeling Factor P250; BRG1-Associated Factor 250; OSA1 Nuclear Protein; Brain Protein 120; C10rf4; C10RF4; C1ORF4; BM029; MRD14; CSS2; ELD
Background

Gene Name: ARID1A

NCBI Gene Entry: 8289

UniProt Entry: O14497
Application Information

Molecular Weight: Predicted, 242 kDa, observed, 270 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB3230

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human ARID1A
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of ARID1A expression in C2C12 cells using ARID1A antibody (Cat#61580, 1:2000). Green, isotype control; red, ARID1A.
  • Immunocytochemical staining of C2C12 cells with ARID1A antibody (Cat#61580, 1:1,000). Nuclei were stained blue with DAPI; ARID1A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
  • Western blotting analysis using anti-ARID1A antibody (Cat#61580). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ARID1A antibody (Cat#61580, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using anti-ARID1A antibody (Cat#61580). ARID1A expression in wild type (WT) and ARID1A shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-ARID1A antibody (Cat#61580, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Validation of ARID1A knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-ARID1A antibody (Cat#61580, 1:2,000) and analyzed using BD flow cytometer.
  • Immunocytochemical staining of Hela cells using anti-ARID1A antibody (Cat#61580, 1:1,000), Top panel: wild-type (WT); Bottom panal: ARID1A shRNA knockdown (KD). Nuclei were stained blue with DAPI; ARID1A was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
Citation(0)
  • 基因名：
    ARID1A
  • 货号：
    61580
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00
RELATED PRODUCTS
Flow cytometric analysis of ARID1A expression in C2C12 cells using ARID1A antibody (Cat#61580, 1:2000). Green, isotype control; red, ARID1A.
Immunocytochemical staining of C2C12 cells with ARID1A antibody (Cat#61580, 1:1,000). Nuclei were stained blue with DAPI; ARID1A was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-ARID1A antibody (Cat#61580). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-ARID1A antibody (Cat#61580, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-ARID1A antibody (Cat#61580). ARID1A expression in wild type (WT) and ARID1A shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-ARID1A antibody (Cat#61580, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Validation of ARID1A knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-ARID1A antibody (Cat#61580, 1:2,000) and analyzed using BD flow cytometer.
Immunocytochemical staining of Hela cells using anti-ARID1A antibody (Cat#61580, 1:1,000), Top panel: wild-type (WT); Bottom panal: ARID1A shRNA knockdown (KD). Nuclei were stained blue with DAPI; ARID1A was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
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