Flow cytometric analysis of NFKB inhibitor alpha expression in HepG2 cells using anti-NFKB inhibitor alpha antibody (Cat#63464, 1:2,000). Green, isotype control; red, NFKB inhibitor alpha.
Immunocytochemical staining of HepG2 cells with anti-NFKB inhibitor alpha antibody (Cat#63464, 1:1,000) . Nuclei were stained blue with DAPI; NFKB inhibitor alpha was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain：Low. Scale bar, 20 μm.
Western blotting analysis using anti-NFKB inhibitor alpha antibody (Cat#63464). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-NFKB inhibitor alpha antibody (Cat#63464, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit(Cat#716).
Western blotting analysis using anti-NFKB inhibitor alpha antibody (Cat#63464). NFKB inhibitor alpha expression in wild type (WT) and NFKB inhibitor alpha (NFKBIA) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-NFKB inhibitor alpha antibody (Cat#63464, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Validation of NFKB inhibitor alpha knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-NFKB inhibitor alpha antibody (Cat#63464, 1:2,000) and analyzed using BD flow cytometer.
