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KD-Validated Anti-PDCD6IP Rabbit Monoclonal Antibody #61605

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Flow cytometric analysis of PDCD6IP expression in HepG2 cells using anti-PDCD6IP antibody (Cat#61605, 1:2,000). Green, isotype control; red, PDCD6IP.
Immunocytochemical staining of HepG2 cells with anti-PDCD6IP antibody (Cat#61605, 1:1,000). Nuclei were stained blue with DAPI; PDCD6IP was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-PDCD6IP antibody (Cat#61605). Total cell lysates (20 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PDCD6IP antibody (Cat#61605, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-PDCD6IP antibody (Cat#61605). PDCD6IP expression in wild-type (WT) and PDCD6IP knockdown (KD) 293T cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-PDCD6IP antibody (Cat#61605, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • 基因名：
    PDCD6IP
  • 货号：
    61605
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information
Product Name KD-Validated Anti-PDCD6IP Rabbit Monoclonal Antibody
Aliases PDCD6IP; Programmed Cell Death 6 Interacting Protein; AIP1; Hp95; Programmed Cell Death 6-Interacting Protein; Alix; ALG-2 Interacting Protein X; PDCD6-Interacting Protein; Apoptosis-Linked Gene 2-Interacting Protein X; Dopamine Receptor Interacting Protein 4; ALG-2 Interacting Protein X; ALG-2 Interacting Protein 1; ALG-2-Interacting Protein 1; KIAA1375; MCPH29; DRIP4; ALIX
Background

Gene Name: PDCD6IP

NCBI Gene Entry: 10015

UniProt Entry: Q8WUM4
Application Information

Molecular Weight: Predicted, 96 kDa; observed, 97 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB3000

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human ALIX
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of PDCD6IP expression in HepG2 cells using anti-PDCD6IP antibody (Cat#61605, 1:2,000). Green, isotype control; red, PDCD6IP.
  • Immunocytochemical staining of HepG2 cells with anti-PDCD6IP antibody (Cat#61605, 1:1,000). Nuclei were stained blue with DAPI; PDCD6IP was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
  • Western blotting analysis using anti-PDCD6IP antibody (Cat#61605). Total cell lysates (20 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PDCD6IP antibody (Cat#61605, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using anti-PDCD6IP antibody (Cat#61605). PDCD6IP expression in wild-type (WT) and PDCD6IP knockdown (KD) 293T cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-PDCD6IP antibody (Cat#61605, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Citation(0)
  • 基因名：
    PDCD6IP
  • 货号：
    61605
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00
RELATED PRODUCTS
Flow cytometric analysis of PDCD6IP expression in HepG2 cells using anti-PDCD6IP antibody (Cat#61605, 1:2,000). Green, isotype control; red, PDCD6IP.
Immunocytochemical staining of HepG2 cells with anti-PDCD6IP antibody (Cat#61605, 1:1,000). Nuclei were stained blue with DAPI; PDCD6IP was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-PDCD6IP antibody (Cat#61605). Total cell lysates (20 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PDCD6IP antibody (Cat#61605, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-PDCD6IP antibody (Cat#61605). PDCD6IP expression in wild-type (WT) and PDCD6IP knockdown (KD) 293T cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-PDCD6IP antibody (Cat#61605, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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