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KD-Validated Anti-BUB1 Rabbit Monoclonal Antibody #61624

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Western blotting analysis using anti-BUB1 antibody (Cat#61624). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-BUB1 antibody (Cat#61624, 1:5,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-BUB1 antibody (Cat#61624). BUB1 expression in wild type (WT) and BUB1 knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-BUB1 antibody (Cat#61624, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • 基因名:
    BUB1
  • 货号:
    61624
  • 应用:
    WB
  • 物种反应性:
    H
规格:
20 μL,50 μL,100 μL
价格:
¥ 650.00,¥ 1,350.00,¥ 2,500.00
Information
Product Name KD-Validated Anti-BUB1 Rabbit Monoclonal Antibody
Aliases BUB1; BUB1 Mitotic Checkpoint Serine/Threonine Kinase; HBUB1; BUB1A; BUB1L; Mitotic Checkpoint Serine/Threonine-Protein Kinase BUB1; Budding Uninhibited By Benzimidazoles 1 (Yeast Homolog); Budding Uninhibited By Benzimidazoles 1 Homolog (Yeast); BUB1 Budding Uninhibited By Benzimidazoles 1 Homolog; Budding Uninhibited By Benzimidazoles 1 Homolog; Putative Serine/Threonine-Protein Kinase; Mitotic Spindle Checkpoint Kinase; EC 2.7.11.1; MCPH30
Background

Gene Name: BUB1

NCBI Gene Entry: 699

UniProt Entry: O43683

Application Information

Molecular Weight: Predicted, 122 kDa, observed, 130 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB3065

Species Reactivity: Human

Applications Tested: Western blotting (WB)

Immunogen A synthesized peptide derived from human Bub1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western blotting (WB): 1:1,000-1:5,000
Note This product is for research use only.
Picture
  • Western blotting analysis using anti-BUB1 antibody (Cat#61624). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-BUB1 antibody (Cat#61624, 1:5,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using anti-BUB1 antibody (Cat#61624). BUB1 expression in wild type (WT) and BUB1 knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-BUB1 antibody (Cat#61624, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Citation(0)
Western blotting analysis using anti-BUB1 antibody (Cat#61624). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-BUB1 antibody (Cat#61624, 1:5,000) and HRP-conjugated goat anti rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-BUB1 antibody (Cat#61624). BUB1 expression in wild type (WT) and BUB1 knockdown (KD) HSHC cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-BUB1 antibody (Cat#61624, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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