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KD-Validated Anti-Superoxide Dismutase 2 Rabbit Monoclonal Antibody #62706

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Immunocytochemical staining of C2C12 cells with anti-Superoxide dismutase 2 antibody (Cat#62706, 1:1,000) . Nuclei were stained blue with DAPI; Superoxide dismutase 2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain: Low. Scale bar, 20 μm.
Western blotting analysis using anti-Superoxide dismutase 2 antibody (Cat#62706). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Superoxide dismutase 2 antibody (Cat#62706, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-Superoxide dismutase 2 antibody (Cat#62706). Superoxide dismutase 2 expression in wild type (WT) and Superoxide dismutase 2 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Superoxide dismutase 2 antibody (Cat#62706, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • 基因名：
    SOD2
  • 货号：
    62706
  • 应用：
    WB, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information
Product Name KD-Validated Anti-Superoxide Dismutase 2 Rabbit Monoclonal Antibody
Aliases SOD2; Superoxide Dismutase 2; GClnc1; MnSOD; IPOB; GC1; Superoxide Dismutase [Mn], Mitochondrial; Superoxide Dismutase 2, Mitochondrial; EC 1.15.1.1; Epididymis Secretory Sperm Binding Protein; Manganese-Containing Superoxide Dismutase; Gastric Cancer–Associated LncRNA 1; Gastric Cancer-Associated LncRNA 1; Manganese Superoxide Dismutase; Mangano-Superoxide Dismutase; Mn Superoxide Dismutase; Indophenoloxidase B; Mn-SOD; IPO-B; MVCD6
Background

Gene Name: SOD2

NCBI Gene Entry: 6648

UniProt Entry: P04179
Application Information

Molecular Weight: Predicted, 25 kDa, observed, 25 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB2135

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human SOD2
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Immunocytochemical staining of C2C12 cells with anti-Superoxide dismutase 2 antibody (Cat#62706, 1:1,000) . Nuclei were stained blue with DAPI; Superoxide dismutase 2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain: Low. Scale bar, 20 μm.
  • Western blotting analysis using anti-Superoxide dismutase 2 antibody (Cat#62706). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Superoxide dismutase 2 antibody (Cat#62706, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using anti-Superoxide dismutase 2 antibody (Cat#62706). Superoxide dismutase 2 expression in wild type (WT) and Superoxide dismutase 2 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Superoxide dismutase 2 antibody (Cat#62706, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Citation(0)
  • 基因名：
    SOD2
  • 货号：
    62706
  • 应用：
    WB, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00
RELATED PRODUCTS
Immunocytochemical staining of C2C12 cells with anti-Superoxide dismutase 2 antibody (Cat#62706, 1:1,000) . Nuclei were stained blue with DAPI; Superoxide dismutase 2 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain: Low. Scale bar, 20 μm.
Western blotting analysis using anti-Superoxide dismutase 2 antibody (Cat#62706). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Superoxide dismutase 2 antibody (Cat#62706, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-Superoxide dismutase 2 antibody (Cat#62706). Superoxide dismutase 2 expression in wild type (WT) and Superoxide dismutase 2 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Superoxide dismutase 2 antibody (Cat#62706, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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