Immunocytochemical staining of HT-1080 cells with anti-Phospho-STAT3 (Tyr705) antibody (Cat#61715, 1:1,000) . Nuclei were stained blue with DAPI; Phospho-STAT3 (Tyr705) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-Phospho-STAT3 (Tyr705) antibody (Cat#61715). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Phospho-STAT3 (Tyr705) antibody (Cat#61715, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-Phospho-STAT3 (Tyr705) antibody (Cat#61715). Phospho-STAT3 (Tyr705) expression in wild type (WT) and Phospho-STAT3 (Tyr705) shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Phospho-STAT3 (Tyr705) antibody (Cat#61715, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
STAT3; Signal Transducer And Activator Of Transcription 3; APRF; Acute-Phase Response Factor; Signal Transducer And Activator Of Transcription 3 (Acute-Phase Response Factor); DNA-Binding Protein APRF; ADMIO1; ADMIO; HIES
Applications Tested: Western blotting (WB), immunocytochemistry (IC)
Immunogen
A synthesized peptide derived from human STAT3
Isotype
Rabbit IgG
Storage Buffer
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for one year.
Recommended Dilutions
Western Blotting (WB): 1:1,000-1:5,000 Immunocytochemistry (IC): 1:100-1:1,000
Note
This product is for research use only.
Picture
Immunocytochemical staining of HT-1080 cells with anti-Phospho-STAT3 (Tyr705) antibody (Cat#61715, 1:1,000) . Nuclei were stained blue with DAPI; Phospho-STAT3 (Tyr705) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-Phospho-STAT3 (Tyr705) antibody (Cat#61715). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Phospho-STAT3 (Tyr705) antibody (Cat#61715, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-Phospho-STAT3 (Tyr705) antibody (Cat#61715). Phospho-STAT3 (Tyr705) expression in wild type (WT) and Phospho-STAT3 (Tyr705) shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Phospho-STAT3 (Tyr705) antibody (Cat#61715, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HT-1080 cells with anti-Phospho-STAT3 (Tyr705) antibody (Cat#61715, 1:1,000) . Nuclei were stained blue with DAPI; Phospho-STAT3 (Tyr705) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-Phospho-STAT3 (Tyr705) antibody (Cat#61715). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Phospho-STAT3 (Tyr705) antibody (Cat#61715, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-Phospho-STAT3 (Tyr705) antibody (Cat#61715). Phospho-STAT3 (Tyr705) expression in wild type (WT) and Phospho-STAT3 (Tyr705) shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Phospho-STAT3 (Tyr705) antibody (Cat#61715, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
