Immunocytochemical staining of HAP-1 cells with HRAS antibody (Cat#63452, 1:1,000) . Nuclei were stained blue with DAPI; HRAS was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: . Scale bar, 20 μm.
Western blotting analysis using anti-HRAS antibody (Cat#63452). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-HRAS antibody (Cat#63452, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-HRAS antibody (Cat#63452). HRAS expression in wild type (WT) and HRAS shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-HRAS antibody (Cat#63452, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Immunocytochemical staining of HeLa cells using anti-HRAS antibody (Cat#63452, 1:1,000), Top panel: wild-type (WT); Bottom panal: HRAS shRNA knockdown (KD). Nuclei were stained blue with DAPI; HRAS was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
