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KD-Validated Anti-Histone H3 (acetyl K56) Rabbit Monoclonal Antibody #61772

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Flow cytometric analysis of Histone H3 (acetyl K56) expression in HAP-1 cells using Histone H3 (acetyl K56) antibody (Cat#61772, 1:2,000). Green, isotype control; red, Histone H3 (acetyl K56).
Immunocytochemical staining of HAP-1 cells with Histone H3 (acetyl K56) antibody (Cat#61772, 1:1,000). Nuclei were stained blue with DAPI; Histone H3 (acetyl K56) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-Histone H3 (acetyl K56) antibody (Cat#61772). Total cell lysates (5 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Histone H3 (acetyl K56) antibody (Cat#61772, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-Histone H3 (acetyl K56) antibody (Cat#61772). Histone H3 (acetyl K56) expression in wild type (WT) and Histone H3 (acetyl K56) shRNA knockdown (KD) HeLa cells with 5 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Histone H3 (acetyl K56) antibody (Cat#61772, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名：
    H3C1
  • 货号：
    61772
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information
Product Name KD-Validated Anti-Histone H3 (acetyl K56) Rabbit Monoclonal Antibody
Aliases H3C1; H3 Clustered Histone 1; HIST1H3A; H3/A; H3FA; Histone Cluster 1 H3 Family Member A; H3 Histone Family, Member A; Histone Cluster 1, H3a; Histone 1, H3a; Histone H3.1; Histone H3/A; H3FC HIST1H3C; Histone H3/B; Histone H3/C; Histone H3/D; Histone H3/F; Histone H3/H; Histone H3/I; Histone; H3/J; Histone H3/K; Histone H3/L; HIST1H3B; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J; H3C10; H3C11; H3C12; H3C2; H3C3; H3C4; H3C6; H3C7; H3C8; H3FL; H3FB; H3FD; H3FI; H3FH; H3FK; H3FF; H3FJ
Background

Gene Name: H3C1

NCBI Gene Entry: 8350

UniProt Entry: P68431
Application Information

Molecular Weight: Predicted, 15 kDa, observed, 15 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB4395

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human Histone H3 (acetyl K56)
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of Histone H3 (acetyl K56) expression in HAP-1 cells using Histone H3 (acetyl K56) antibody (Cat#61772, 1:2,000). Green, isotype control; red, Histone H3 (acetyl K56).
  • Immunocytochemical staining of HAP-1 cells with Histone H3 (acetyl K56) antibody (Cat#61772, 1:1,000). Nuclei were stained blue with DAPI; Histone H3 (acetyl K56) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
  • Western blotting analysis using anti-Histone H3 (acetyl K56) antibody (Cat#61772). Total cell lysates (5 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Histone H3 (acetyl K56) antibody (Cat#61772, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using anti-Histone H3 (acetyl K56) antibody (Cat#61772). Histone H3 (acetyl K56) expression in wild type (WT) and Histone H3 (acetyl K56) shRNA knockdown (KD) HeLa cells with 5 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Histone H3 (acetyl K56) antibody (Cat#61772, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
  • 基因名：
    H3C1
  • 货号：
    61772
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00
RELATED PRODUCTS
Flow cytometric analysis of Histone H3 (acetyl K56) expression in HAP-1 cells using Histone H3 (acetyl K56) antibody (Cat#61772, 1:2,000). Green, isotype control; red, Histone H3 (acetyl K56).
Immunocytochemical staining of HAP-1 cells with Histone H3 (acetyl K56) antibody (Cat#61772, 1:1,000). Nuclei were stained blue with DAPI; Histone H3 (acetyl K56) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-Histone H3 (acetyl K56) antibody (Cat#61772). Total cell lysates (5 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Histone H3 (acetyl K56) antibody (Cat#61772, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-Histone H3 (acetyl K56) antibody (Cat#61772). Histone H3 (acetyl K56) expression in wild type (WT) and Histone H3 (acetyl K56) shRNA knockdown (KD) HeLa cells with 5 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Histone H3 (acetyl K56) antibody (Cat#61772, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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