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KD-Validated Anti-Phospho-c-Jun (T91) Rabbit Monoclonal Antibody #61795

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Flow cytometric analysis of Phospho-c-Jun (T91) expression in C2C12 cells using Phospho-c-Jun (T91) antibody (Cat#61795, 1:2,000). Green, isotype control; red, Phospho-c-Jun (T91).
Immunocytochemical staining of C2C12 cells with anti-Phospho-c-Jun (T91) antibody (Cat#61795, 1:1,000) . Nuclei were stained blue with DAPI; Phospho-c-Jun (T91) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-Phospho-c-Jun (T91) antibody (Cat#61795). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Phospho-c-Jun (T91) antibody (Cat#61795, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-phospho-c-Jun (T91) antibody (Cat#61795). Phospho-c-Jun (T91) expression in wild-type (WT) and JUN shRNA knockdown (KD) C2C12 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-phospho-c-Jun (T91) antibody (Cat#61795, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). JUN, Jun proto-oncogene, AP-1 transcription factor subunit.
  • 基因名：
    JUN
  • 货号：
    61795
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information
Product Name KD-Validated Anti-Phospho-c-Jun (T91) Rabbit Monoclonal Antibody
Aliases JUN; Jun Proto-Oncogene, AP-1 Transcription Factor Subunit; V-Jun Avian Sarcoma Virus 17 Oncogene Homolog; AP-1; Transcription Factor AP-1 Subunit Jun; Transcription Factor Jun; Proto-Oncogene C-Jun; Activator Protein 1; Jun Oncogene; C-Jun; AP1; P39; V-Jun Sarcoma Virus 17 Oncogene Homolog; Jun Activation Domain Binding Protein; Enhancer-Binding Protein AP1; Transcription Factor AP-1; Proto-Oncogene CJun; C-JUN; CJUN
Background

Gene Name: JUN

NCBI Gene Entry: 3725

UniProt Entry: P05412
Application Information

Molecular Weight: Predicted, 36 kDa, observed, 43 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB4500

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human c-Jun
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of Phospho-c-Jun (T91) expression in C2C12 cells using Phospho-c-Jun (T91) antibody (Cat#61795, 1:2,000). Green, isotype control; red, Phospho-c-Jun (T91).
  • Immunocytochemical staining of C2C12 cells with anti-Phospho-c-Jun (T91) antibody (Cat#61795, 1:1,000) . Nuclei were stained blue with DAPI; Phospho-c-Jun (T91) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
  • Western blotting analysis using anti-Phospho-c-Jun (T91) antibody (Cat#61795). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Phospho-c-Jun (T91) antibody (Cat#61795, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using anti-phospho-c-Jun (T91) antibody (Cat#61795). Phospho-c-Jun (T91) expression in wild-type (WT) and JUN shRNA knockdown (KD) C2C12 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-phospho-c-Jun (T91) antibody (Cat#61795, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). JUN, Jun proto-oncogene, AP-1 transcription factor subunit.
Citation(0)
  • 基因名：
    JUN
  • 货号：
    61795
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00
RELATED PRODUCTS
Flow cytometric analysis of Phospho-c-Jun (T91) expression in C2C12 cells using Phospho-c-Jun (T91) antibody (Cat#61795, 1:2,000). Green, isotype control; red, Phospho-c-Jun (T91).
Immunocytochemical staining of C2C12 cells with anti-Phospho-c-Jun (T91) antibody (Cat#61795, 1:1,000) . Nuclei were stained blue with DAPI; Phospho-c-Jun (T91) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-Phospho-c-Jun (T91) antibody (Cat#61795). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Phospho-c-Jun (T91) antibody (Cat#61795, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-phospho-c-Jun (T91) antibody (Cat#61795). Phospho-c-Jun (T91) expression in wild-type (WT) and JUN shRNA knockdown (KD) C2C12 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-phospho-c-Jun (T91) antibody (Cat#61795, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716). JUN, Jun proto-oncogene, AP-1 transcription factor subunit.
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