Western blotting analysis using anti-fumarate hydratase antibody (Cat#61980). Total cell lysates (5 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-fumarate hydratase antibody (Cat#61980, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-fumarate hydratase antibody (Cat#61980). Fumarate hydratase expression in wild-type (WT) and fumarate hydratase (FH) shRNA knockdown (KD) HepG2 cells with 5 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-fumarate hydratase antibody (Cat#61980, 1:2,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Validation of Fumarate hydratase knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-Fumarate hydratase antibody (Cat#61980, 1:2,000) and analyzed using BD flow cytometer.
