Flow cytometric analysis of Glucose-6-phosphate isomerase expression in HeLa cells using Glucose-6-phosphate isomerase antibody (Cat#62019, 1:2,000). Green, isotype control; red, Glucose-6-phosphate isomerase.
Immunocytochemical staining of Hela cells with anti-Glucose-6-phosphate isomerase antibody (Cat #62019, 1:1,000) . Nuclei were stained blue with DAPI; Glucose-6-phosphate isomerase was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Western blotting analysis using anti-Glucose-6-phosphate isomerase antibody (Cat#62019). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Glucose-6-phosphate isomerase antibody (Cat#62019, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-Glucose 6 phosphate isomerase antibody (Cat#62019). Glucose 6 phosphate isomerase expression in wild type (WT) and Glucose 6 phosphate isomerase shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Glucose 6 phosphate isomerase antibody (Cat#62019, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Validation of Glucose-6-phosphate isomerase knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-Glucose-6-phosphate isomerase antibody (Cat#62019, 1:2,000) and analyzed using BD flow cytometer.
