Flow cytometric analysis of SAMHD1 expression in HeLa cells using SAMHD1 antibody (Cat#62473, 1:2,000). Green, isotype control; red, SAMHD1.
Immunocytochemical staining of Hela cells with anti-SAMHD1 antibody (Cat#62473, 1:1,000). Nuclei were stained blue with DAPI; SAMHD1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
Western blotting analysis using anti-SAMHD1 antibody (Cat#62473). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-SAMHD1 antibody (Cat#62473, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-SAMHD1 antibody (Cat#62473). SAMHD1 expression in wild-type (WT) and SAMHD1 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-SAMHD1 antibody (Cat#62473, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Validation of SAMHD1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-SAMHD1 antibody (Cat#62473, 1:2,000) and analyzed using BD flow cytometer.
Immunocytochemical staining of HeLa cells using anti-SAMHD1 antibody (Cat#62473, 1:1,000), Top panel: wild-type (WT); Bottom panal: SAMHD1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; GRK2 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.
