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KD-Validated Anti-RBBP4 Rabbit Monoclonal Antibody #62522

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Flow cytometric analysis of RBBP4 expression in HepG2 cells using anti-RBBP4 antibody (Cat#62522, 1:2,000). Green, isotype control; red, RBBP4.
Immunocytochemical staining of HepG2 cells with anti-RBBP4 antibody (Cat#62522, 1:1,000). Nuclei were stained blue with DAPI; RBBP4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-RBBP4 antibody (Cat#62522). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-RBBP4 antibody (Cat#62522, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-RBBP4 antibody (Cat#62522). RBBP4 expression in wild type (WT) and RBBP4 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-RBBP4 antibody (Cat#62522, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Validation of RBBP4 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-RBBP4 antibody (Cat#62522, 1:2,000) and analyzed using BD flow cytometer.
Immunocytochemical staining of HeLa cells using anti-RBBP4 antibody (Cat#62522, 1:1,000), Top panel: wild-type (WT); Bottom panal: RBBP4 shRNA knockdown (KD). Nuclei were stained blue with DAPI; RBBP4 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.
Immunohistochemistry was performed on paraffin-embedded mouse liver using anti-RBBP4 antibody (Cat#62522, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunohistochemistry was performed on paraffin-embedded mouse kidney using anti-RBBP4 antibody (Cat#62522, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunohistochemistry was performed on paraffin-embedded mouse brain using anti-RBBP4 antibody (Cat#62522, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunohistochemistry was performed on paraffin-embedded human breast carcinoma using anti-RBBP4 antibody (Cat#62522, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
  • 基因名：
    RBBP4
  • 货号：
    62522
  • 应用：
    WB, FCM, IC, IHC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information
Product Name KD-Validated Anti-RBBP4 Rabbit Monoclonal Antibody
Aliases RB Binding Protein 4, Chromatin Remodeling Factor; Retinoblastoma-Binding Protein 4; RbAp48; NURF55; Lin-53; Nucleosome-Remodeling Factor Subunit RBAP48; Chromatin Assembly Factor I P48 Subunit; Chromatin Assembly Factor 1 Subunit C; Retinoblastoma-Binding Protein P48; Histone-Binding Protein RBBP4; CAF-I 48 KDa Subunit; CAF-1 Subunit C; CAF-I P48; RBBP-4; Chromatin Assembly Factor/CAF-1 P48 Subunit; Retinoblastoma Binding Protein 4; MSI1 Protein Homolog; RBAP48
Background

Gene Name: RBBP4

NCBI Gene Entry: 5928

UniProt Entry: Q09028
Application Information

Molecular Weight: Predicted, 48 kDa, observed, 52 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB1240

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC), immunohistochemistry (IHC)
Immunogen A synthesized peptide derived from human RbAp48
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Immunohistochemistry (IHC): 1:100-1:200
Note This product is for research use only.
Picture
  • Flow cytometric analysis of RBBP4 expression in HepG2 cells using anti-RBBP4 antibody (Cat#62522, 1:2,000). Green, isotype control; red, RBBP4.
  • Immunocytochemical staining of HepG2 cells with anti-RBBP4 antibody (Cat#62522, 1:1,000). Nuclei were stained blue with DAPI; RBBP4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
  • Western blotting analysis using anti-RBBP4 antibody (Cat#62522). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-RBBP4 antibody (Cat#62522, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using anti-RBBP4 antibody (Cat#62522). RBBP4 expression in wild type (WT) and RBBP4 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-RBBP4 antibody (Cat#62522, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Validation of RBBP4 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-RBBP4 antibody (Cat#62522, 1:2,000) and analyzed using BD flow cytometer.
  • Immunocytochemical staining of HeLa cells using anti-RBBP4 antibody (Cat#62522, 1:1,000), Top panel: wild-type (WT); Bottom panal: RBBP4 shRNA knockdown (KD). Nuclei were stained blue with DAPI; RBBP4 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.
  • Immunohistochemistry was performed on paraffin-embedded mouse liver using anti-RBBP4 antibody (Cat#62522, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
  • Immunohistochemistry was performed on paraffin-embedded mouse kidney using anti-RBBP4 antibody (Cat#62522, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
  • Immunohistochemistry was performed on paraffin-embedded mouse brain using anti-RBBP4 antibody (Cat#62522, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
  • Immunohistochemistry was performed on paraffin-embedded human breast carcinoma using anti-RBBP4 antibody (Cat#62522, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Citation(0)
  • 基因名：
    RBBP4
  • 货号：
    62522
  • 应用：
    WB, FCM, IC, IHC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00
RELATED PRODUCTS
Flow cytometric analysis of RBBP4 expression in HepG2 cells using anti-RBBP4 antibody (Cat#62522, 1:2,000). Green, isotype control; red, RBBP4.
Immunocytochemical staining of HepG2 cells with anti-RBBP4 antibody (Cat#62522, 1:1,000). Nuclei were stained blue with DAPI; RBBP4 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-RBBP4 antibody (Cat#62522). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-RBBP4 antibody (Cat#62522, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-RBBP4 antibody (Cat#62522). RBBP4 expression in wild type (WT) and RBBP4 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-RBBP4 antibody (Cat#62522, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Validation of RBBP4 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-RBBP4 antibody (Cat#62522, 1:2,000) and analyzed using BD flow cytometer.
Immunocytochemical staining of HeLa cells using anti-RBBP4 antibody (Cat#62522, 1:1,000), Top panel: wild-type (WT); Bottom panal: RBBP4 shRNA knockdown (KD). Nuclei were stained blue with DAPI; RBBP4 was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm. Permeabilization: Triton.
Immunohistochemistry was performed on paraffin-embedded mouse liver using anti-RBBP4 antibody (Cat#62522, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunohistochemistry was performed on paraffin-embedded mouse kidney using anti-RBBP4 antibody (Cat#62522, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunohistochemistry was performed on paraffin-embedded mouse brain using anti-RBBP4 antibody (Cat#62522, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
Immunohistochemistry was performed on paraffin-embedded human breast carcinoma using anti-RBBP4 antibody (Cat#62522, 1:200). Antigen retrieval was done in sodium citrate buffer (pH 6.0). DAB was used for detection, with hematoxylin counterstaining. Images were acquired using a Nikon Ci-L Plus microscope (40× objective). Scale bar: 25 μm.
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