Immunocytochemical staining of C2C12 cells with anti-DNA polymerase beta antibody (Cat#62575, 1:1,000) . Nuclei were stained blue with DAPI; DNA polymerase beta was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain：Low. Scale bar, 20 μm.
Western blotting analysis using anti-DNA polymerase beta antibody (Cat#62575). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-DNA polymerase beta antibody (Cat#62575, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-DNA Polymerase beta antibody (Cat#62575). DNA Polymerase beta expression in wild-type (WT) and DNA Polymerase beta (POLB) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-DNA Polymerase beta antibody (Cat#62575, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Validation of DNA polymerase beta knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-DNA polymerase beta antibody (Cat#62575, 1:2,000) and analyzed using BD flow cytometer.
