Flow cytometric analysis of beta Tubulin III expression in HT-1080 cells using anti-beta Tubulin III antibody (Cat#62635, 1:2,000). Green, isotype control; red, beta Tubulin III.
Immunocytochemical staining of HT-1080 cells with anti-beta Tubulin III antibody (Cat#62635, 1:1,000) . Nuclei were stained blue with DAPI; beta Tubulin III was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-beta Tubulin III antibody (Cat#62635). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-beta Tubulin III antibody (Cat#62635, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-beta Tubulin III antibody (Cat#62635). beta Tubulin III expression in wild type (WT) and beta Tubulin III shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-beta Tubulin III antibody (Cat#62635, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Validation of beta Tubulin III knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-beta Tubulin III antibody (Cat#62635, 1:2,000) and analyzed using BD flow cytometer.
Immunocytochemical staining of Hela cells using anti-Beta Tubulin III antibody (Cat#62635, 1:1,000), Top panel: wild-type (WT); Bottom panal: Beta Tubulin III shRNA knockdown (KD). Nuclei were stained blue with DAPI; Beta Tubulin III was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
