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Anti-Protein Tyrosine Kinase 2 Beta Rabbit Monoclonal Antibody #2503

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Flow cytometric analysis of Protein tyrosine kinase 2 beta expression in H9c2 cells using anti-Protein tyrosine kinase 2 beta antibody (Cat#2503, 1:2,000). Green, isotype control; red, Protein tyrosine kinase 2 beta.
Immunocytochemical staining of H9C2 cells with anti-Protein tyrosine kinase 2 beta antibody (Cat#2503, 1:1,000) . Nuclei were stained blue with DAPI;Protein tyrosine kinase 2 beta was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-Protein tyrosine kinase 2 beta antibody (Cat#2503). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Protein tyrosine kinase 2 beta antibody (Cat#2503, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名:
    PTK2B
  • 货号:
    2503
  • 应用:
    WB, FCM, IC
  • 物种反应性:
    H, M, R
规格:
20 μL,50 μL,100 μL
价格:
¥ 620.00,¥ 1,200.00,¥ 2,200.00
Information
Product Name Anti-Protein Tyrosine Kinase 2 Beta Rabbit Monoclonal Antibody
Aliases Protein Tyrosine Kinase 2 Beta; RAFTK; CADTK; CAKB; PYK2; FAK2; PTK; Calcium-Regulated Non-Receptor Proline-Rich Tyrosine Kinase; Related Adhesion Focal Tyrosine Kinase; Calcium-Dependent Tyrosine Kinase; Protein-Tyrosine Kinase 2-Beta; Proline-Rich Tyrosine Kinase 2; Cell Adhesion Kinase Beta; Focal Adhesion Kinase 2; EC 2.7.10.2; CAK-Beta; FADK 2; PTK2B Protein Tyrosine Kinase 2 Beta; Protein Kinase B; EC 2.7.10; FADK2; PKB
Background

Gene Name: PTK2B

NCBI Gene Entry: 2185

UniProt Entry: Q14289

Application Information

Molecular Weight: Predicted, 116 kDa; observed, 116 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 24GB1155

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)

Immunogen A synthesized peptide derived from human PYK2
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of Protein tyrosine kinase 2 beta expression in H9c2 cells using anti-Protein tyrosine kinase 2 beta antibody (Cat#2503, 1:2,000). Green, isotype control; red, Protein tyrosine kinase 2 beta.
  • Immunocytochemical staining of H9C2 cells with anti-Protein tyrosine kinase 2 beta antibody (Cat#2503, 1:1,000) . Nuclei were stained blue with DAPI;Protein tyrosine kinase 2 beta was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
  • Western blotting analysis using anti-Protein tyrosine kinase 2 beta antibody (Cat#2503). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Protein tyrosine kinase 2 beta antibody (Cat#2503, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
Flow cytometric analysis of Protein tyrosine kinase 2 beta expression in H9c2 cells using anti-Protein tyrosine kinase 2 beta antibody (Cat#2503, 1:2,000). Green, isotype control; red, Protein tyrosine kinase 2 beta.
Immunocytochemical staining of H9C2 cells with anti-Protein tyrosine kinase 2 beta antibody (Cat#2503, 1:1,000) . Nuclei were stained blue with DAPI;Protein tyrosine kinase 2 beta was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-Protein tyrosine kinase 2 beta antibody (Cat#2503). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Protein tyrosine kinase 2 beta antibody (Cat#2503, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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