Western blotting analysis using anti-leukotriene A4 hydrolase antibody (Cat#63418). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-leukotriene A4 hydrolase antibody (Cat#63418, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit(Cat#716).
Western blotting analysis using anti-leukotriene A4 hydrolase antibody (Cat#63418). Leukotriene A4 hydrolase expression in wild type (WT) and leukotriene A4 hydrolase (LTA4H) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-leukotriene A4 hydrolase antibody (Cat#63418, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage
Store at -20 °C for one year.
Recommended Dilutions
Western Blotting (WB):1:2,500-1:5,000
Note
This product is for research use only.
Picture
Western blotting analysis using anti-leukotriene A4 hydrolase antibody (Cat#63418). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-leukotriene A4 hydrolase antibody (Cat#63418, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit(Cat#716).
Western blotting analysis using anti-leukotriene A4 hydrolase antibody (Cat#63418). Leukotriene A4 hydrolase expression in wild type (WT) and leukotriene A4 hydrolase (LTA4H) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-leukotriene A4 hydrolase antibody (Cat#63418, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-leukotriene A4 hydrolase antibody (Cat#63418). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-leukotriene A4 hydrolase antibody (Cat#63418, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit(Cat#716).
Western blotting analysis using anti-leukotriene A4 hydrolase antibody (Cat#63418). Leukotriene A4 hydrolase expression in wild type (WT) and leukotriene A4 hydrolase (LTA4H) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-leukotriene A4 hydrolase antibody (Cat#63418, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
