Flow cytometric analysis of CD147 expression in HeLa cells using anti-CD147 antibody (Cat#63423, 1:2000). Green, isotype control; red, CD147.
Immunocytochemical staining of HeLa cells with anti-CD147 antibody (Cat#63423, 1:1,000) . Nuclei were stained blue with DAPI; CD147 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain：Medium. Scale bar, 20 μm.
Western blotting analysis using anti-CD147 antibody (Cat#63423). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-CD147 antibody (Cat#63423, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit(Cat#716).
Western blotting analysis using anti-CD147 antibody (Cat#63423). CD147 expression in wild type (WT) and BSG shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-CD147 antibody (Cat#63423, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
