Immunocytochemical staining of HAP-1 cells with Farnesyl-diphosphate farnesyltransferase 1 antibody (Cat#63442, 1:1,000) . Nuclei were stained blue with DAPI; Farnesyl-diphosphate farnesyltransferase 1 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-farnesyl-diphosphate farnesyltransferase 1 antibody (Cat#63442). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-farnesyl-diphosphate farnesyltransferase 1 antibody (Cat#63442, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit(Cat#716).
Western blotting analysis using anti-farnesyl-diphosphate farnesyltransferase 1 antibody (Cat#63442). Farnesyl-diphosphate farnesyltransferase 1 expression in wild type (WT) and farnesyl-diphosphate farnesyltransferase 1 (FDFT1) shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-farnesyl-diphosphate farnesyltransferase 1 antibody (Cat#63442, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
