Flow cytometric analysis of PFKP expression in HepG2 cells using anti-PFKP antibody (Cat#63536, 1:2,000). Green, isotype control; red, PFKP.
Immunocytochemical staining of HepG2 cells with anti-PFKP antibody (Cat#63536, 1:1,000) . Nuclei were stained blue with DAPI; PFKP was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and Smart Gain：Medium. Scale bar, 20 μm.
Western blotting analysis using anti-PFKP antibody (Cat#63536). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-PFKP antibody (Cat#63536, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-PFKP antibody (Cat#63536). PFKP expression in wild type (WT) and PFKP knockdown (KD) HSHC cells with 20 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-PFKP antibody (Cat#63536, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
