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KD-Validated Anti-APMAP Mouse Monoclonal Antibody #63542

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Flow cytometric analysis of APMAP expression in HT-1080 cells using anti-APMAP antibody (Cat#63542, 1:2,000). Green, isotype control; red, APMAP.
Western blotting analysis using anti-APMAP antibody (Cat#63542). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-APMAP antibody (Cat#63542, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-APMAP antibody (Cat#63542). APMAP expression in wild type (WT) and APMAP shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-APMAP antibody (Cat#63542, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • 基因名:
    APMAP
  • 货号:
    63542
  • 应用:
    WB, FCM
  • 物种反应性:
    H
规格:
20 μL,50 μL,100 μL
价格:
¥ 650.00,¥ 1,350.00,¥ 2,500.00
Information
Product Name KD-Validated Anti-APMAP Mouse Monoclonal Antibody
Aliases APMAP; Adipocyte Plasma Membrane Associated Protein; C20orf3; BSCv; Adipocyte Plasma Membrane-Associated Protein; Protein BSCv; Chromosome 20 Open Reading Frame 3
Background

Gene Name: APMAP

NCBI Gene Entry: 57136

UniProt Entry: Q9HDC9

Application Information

Molecular Weight: Predicted, 46 kDa; observed, 46 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 24GB6435

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM)

Immunogen Recombinant protein of human C20orf3
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of APMAP expression in HT-1080 cells using anti-APMAP antibody (Cat#63542, 1:2,000). Green, isotype control; red, APMAP.
  • Western blotting analysis using anti-APMAP antibody (Cat#63542). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-APMAP antibody (Cat#63542, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using anti-APMAP antibody (Cat#63542). APMAP expression in wild type (WT) and APMAP shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-APMAP antibody (Cat#63542, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Citation(0)
Flow cytometric analysis of APMAP expression in HT-1080 cells using anti-APMAP antibody (Cat#63542, 1:2,000). Green, isotype control; red, APMAP.
Western blotting analysis using anti-APMAP antibody (Cat#63542). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-APMAP antibody (Cat#63542, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-APMAP antibody (Cat#63542). APMAP expression in wild type (WT) and APMAP shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-APMAP antibody (Cat#63542, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
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