Flow cytometric analysis of APMAP expression in HT-1080 cells using anti-APMAP antibody (Cat#63542, 1:2,000). Green, isotype control; red, APMAP.
Western blotting analysis using anti-APMAP antibody (Cat#63542). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-APMAP antibody (Cat#63542, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-APMAP antibody (Cat#63542). APMAP expression in wild type (WT) and APMAP shRNA knockdown (KD) HT-1080 cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-APMAP antibody (Cat#63542, 1:5,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).