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KD-Validated Anti-HADHA Mouse Monoclonal Antibody #63587

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Flow cytometric analysis of HADHA expression in HepG2 cells using anti-HADHA antibody (Cat#63587, 1:2,000). Green, isotype control; red, HADHA.
Western blotting analysis using anti-HADHA antibody (Cat#63587). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-HADHA antibody (Cat#63587, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-HADHA antibody (Cat#63587). HADHA expression in wild type (WT) and HADHA shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-HADHA antibody (Cat#63587, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Validation of HADHA knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-HADHA antibody (Cat#63587, 1:2,000) and analyzed using BD flow cytometer.
  • 基因名:
    HADHA
  • 货号:
    63587
  • 应用:
    WB, FCM
  • 物种反应性:
    H, M, R
规格:
20 μL,50 μL,100 μL
价格:
¥ 650.00,¥ 1,350.00,¥ 2,500.00
Information
Product Name KD-Validated Anti-HADHA Mouse Monoclonal Antibody
Aliases HADHA; Hydroxyacyl-CoA Dehydrogenase Trifunctional Multienzyme Complex Subunit Alpha; LCHAD; LCEH; MTPA; GBP; Hydroxyacyl-Coenzyme A Dehydrogenase/3-Ketoacyl-Coenzyme A Thiolase/Enoyl-Coenzyme A Hydratase (Trifunctional Protein), Alpha Subunit; Hydroxyacyl-CoA Dehydrogenase/3-Ketoacyl-CoA Thiolase/Enoyl-CoA Hydratase (Trifunctional Protein), Alpha Subunit; Mitochondrial Trifunctional Protein, Alpha Subunit; Trifunctional Enzyme Subunit Alpha, Mitochondrial; Long-Chain-3-Hydroxyacyl-CoA Dehydrogenase; Monolysocardiolipin Acyltransferase; Long-Chain 2-Enoyl-CoA Hydratase; 78 KDa Gastrin-Binding Protein; Gastrin-Binding Protein; HADH; Mitochondrial Long-Chain L-3-Hydroxyacyl-Coenzyme A (CoA) Dehydrogenase, Alpha Subunit; Mitochondrial Long-Chain 2-Enoyl-Coenzyme A (CoA) Hydratase, Alpha Subunit; 3-Ketoacyl-Coenzyme A (CoA) Thiolase, Alpha Subunit; Mitochondrial Trifunctional Enzyme, Alpha Subunit; 3-Oxoacyl-CoA Thiolase; EC 2.3.1.- ; TP-ALPHA; TP-Alpha; ECHA
Background

Gene Name: HADHA

NCBI Gene Entry: 3030

UniProt Entry: P40939

Application Information

Molecular Weight: Predicted, 83 kDa; observed, 73 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 24GB6655

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM)

Immunogen Recombinant protein of human HADHA
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:2,500
Flow Cytometry (FCM): 1:2,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of HADHA expression in HepG2 cells using anti-HADHA antibody (Cat#63587, 1:2,000). Green, isotype control; red, HADHA.
  • Western blotting analysis using anti-HADHA antibody (Cat#63587). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-HADHA antibody (Cat#63587, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using anti-HADHA antibody (Cat#63587). HADHA expression in wild type (WT) and HADHA shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-HADHA antibody (Cat#63587, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Validation of HADHA knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-HADHA antibody (Cat#63587, 1:2,000) and analyzed using BD flow cytometer.
Citation(0)
Flow cytometric analysis of HADHA expression in HepG2 cells using anti-HADHA antibody (Cat#63587, 1:2,000). Green, isotype control; red, HADHA.
Western blotting analysis using anti-HADHA antibody (Cat#63587). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-HADHA antibody (Cat#63587, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-HADHA antibody (Cat#63587). HADHA expression in wild type (WT) and HADHA shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-HADHA antibody (Cat#63587, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Validation of HADHA knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-HADHA antibody (Cat#63587, 1:2,000) and analyzed using BD flow cytometer.
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