Flow cytometric analysis of IRAK3 in HAP-1 cells using IRAK3 antibody (Cat#63613, 1:2,000). Green, isotype control; red, IRAK3.
Immunocytochemical staining of HAP-1 cells with IRAK3 antibody (Cat#63613, 1:1,000). Nuclei were stained blue with DAPI; IRAK3 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar: 20 μm.
Western blotting analysis using anti-IRAK3 antibody (Cat#63613). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-IRAK3 antibody (Cat#63613, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-IRAK3 antibody (Cat#63613). IRAK3 expression in wild type (WT) and IRAK3 shRNA knockdown (KD) HT-1080 cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-IRAK3 antibody (Cat#63613, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
