Immunocytochemical staining of HAP1 cells with anti-Epoxide hydrolase 1 antibody (Cat#63860, 1:500) . Nuclei were stained blue with DAPI; Epoxide hydrolase 1 stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Low. Scale bar, 20 μm.
Western blotting analysis using anti-epoxide hydrolase 1 antibody (Cat#63860). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-epoxide hydrolase 1 antibody (Cat#63860, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-epoxide hydrolase 1 antibody (Cat#63860). Epoxide hydrolase 1 expression in wild-type (WT) and epoxide hydrolase 1 (EPHX1) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-epoxide hydrolase 1 antibody (Cat#63860, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
