Flow cytometric analysis of Fumarate hydratase expression in HepG2 cells using anti-Fumarate hydratase antibody (Cat#64801, 1:2,000). Green, isotype control; red, Fumarate hydratase.
Immunocytochemical staining of HepG2 cells with anti-Fumarate hydratase associated protein antibody (Cat#64801, 1:1,000) . Nuclei were stained blue with DAPI; Fumarate hydratase associated protein was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar, 20 μm.
Western blotting analysis using anti-fumarate hydratase antibody (Cat#64801). Total cell lysates (5 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-fumarate hydratase antibody (Cat#64801, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-fumarate hydratase antibody (Cat #64801). Fumarate hydratase expression in wild-type (WT) and fumarate hydratase (FH) shRNA knockdown (KD) HeLa cells with 5 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-fumarate hydratase antibody (Cat #64801, 1:2,500) and HRP-conjugated goat anti-mouse secondary antibody (Cat #101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat #716).
Validation of Fumarate hydratase knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-Fumarate hydratase antibody (Cat#64801, 1:2,000) and analyzed using BD flow cytometer.
Immunocytochemical staining of HeLa cells using anti-Fumarate hydratase antibody (Cat#64801, 1:1,000), Top panel: wild-type (WT); Bottom panal: Fumarate hydratase shRNA knockdown (KD). Nuclei were stained blue with DAPI; V was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
