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KD-Validated Anti-GDI1 Mouse Monoclonal Antibody #64827

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Flow cytometric analysis of GDI1 expression in HAP-1 cells using anti-GDI1 antibody (Cat#64827, 1:2,000). Green, isotype control; red, GDI1.
Western blotting analysis using anti-GDI1 antibody (Cat#64827). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-GDI1 antibody (Cat#64827, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-GDI1 antibody (Cat#64827). GDI1 expression in wild-type (WT) and GDI1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-GDI1 antibody (Cat#64827, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Validation of GDI1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-GDI1 antibody (Cat#64827, 1:2,000) and analyzed using BD flow cytometer.
Immunocytochemical staining of HeLa cells using anti-GDI1 antibody (Cat#64827, 1:1,000), Top panel: wild-type (WT); Bottom panal: GDI1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Acyl-CoA dehydrogenase short chain was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
  • 基因名：
    GDI1
  • 货号：
    64827
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information
Product Name KD-Validated Anti-GDI1 Mouse Monoclonal Antibody
Aliases GDI1; GDP Dissociation Inhibitor 1; RABGDIA; OPHN2; Oligophrenin-2; XAP-4; GDIL; Guanosine Diphosphate Dissociation Inhibitor 1; Rab GDP-Dissociation Inhibitor, Alpha; Rab GDP Dissociation Inhibitor Alpha; Protein XAP-4; Rab GDI Alpha; FLJ41411; MRX41; MRX48; GDI-1; Testis Secretory Sperm-Binding Protein Li 208a; Mental Retardation, X-Linked 41; Mental Retardation, X-Linked 48; RABGD1A; XLID41; XAP4; 1A
Background

Gene Name: GDI1

NCBI Gene Entry: 2664

UniProt Entry: P31150
Application Information

Molecular Weight: Predicted, 51 kDa; observed, 60 kDa

Clonality: Mouse monoclonal antibody

Clone ID: 24GB13140

Species Reactivity: Human

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen Recombinant protein of human GDI1
Isotype Mouse IgG1
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:200-1:1,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of GDI1 expression in HAP-1 cells using anti-GDI1 antibody (Cat#64827, 1:2,000). Green, isotype control; red, GDI1.
  • Western blotting analysis using anti-GDI1 antibody (Cat#64827). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-GDI1 antibody (Cat#64827, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using anti-GDI1 antibody (Cat#64827). GDI1 expression in wild-type (WT) and GDI1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-GDI1 antibody (Cat#64827, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Validation of GDI1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-GDI1 antibody (Cat#64827, 1:2,000) and analyzed using BD flow cytometer.
  • Immunocytochemical staining of HeLa cells using anti-GDI1 antibody (Cat#64827, 1:1,000), Top panel: wild-type (WT); Bottom panal: GDI1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Acyl-CoA dehydrogenase short chain was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
Citation(0)
  • 基因名：
    GDI1
  • 货号：
    64827
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00
RELATED PRODUCTS
Flow cytometric analysis of GDI1 expression in HAP-1 cells using anti-GDI1 antibody (Cat#64827, 1:2,000). Green, isotype control; red, GDI1.
Western blotting analysis using anti-GDI1 antibody (Cat#64827). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-GDI1 antibody (Cat#64827, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-GDI1 antibody (Cat#64827). GDI1 expression in wild-type (WT) and GDI1 shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-GDI1 antibody (Cat#64827, 1:1,000) and HRP-conjugated goat anti-mouse secondary antibody (Cat#101, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Validation of GDI1 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-GDI1 antibody (Cat#64827, 1:2,000) and analyzed using BD flow cytometer.
Immunocytochemical staining of HeLa cells using anti-GDI1 antibody (Cat#64827, 1:1,000), Top panel: wild-type (WT); Bottom panal: GDI1 shRNA knockdown (KD). Nuclei were stained blue with DAPI; Acyl-CoA dehydrogenase short chain was stained magenta with Alexa Fluor® 647. Scale bar, 20 μm.
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