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KD-Validated Anti-Caspase 3 Rabbit Polyclonal Antibody #64941

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Western blotting analysis using anti-caspase 3 antibody (Cat#64941). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-caspase 3 antibody (Cat#64941, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-caspase 3 antibody (Cat #64941). Caspase 3 expression in wild-type (WT) and caspase 3 (CASP3) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-caspase 3 antibody (Cat #64941, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat #201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat #716).
  • 基因名:
    CASP3
  • 货号:
    64941
  • 应用:
    WB
  • 物种反应性:
    H, R
规格:
20 μL,50 μL,100 μL
价格:
¥ 650.00,¥ 1,350.00,¥ 2,500.00
Information
Product Name KD-Validated Anti-Caspase 3 Rabbit Polyclonal Antibody
Aliases CASP3; Caspase 3; Apopain; CPP32; CPP32B; Caspase 3, Apoptosis-Related Cysteine Peptidase; Caspase 3, Apoptosis-Related Cysteine Protease; SREBP Cleavage Activity 1; Cysteine Protease CPP32; Protein Yama; EC 3.4.22.56; Caspase-3; CASP-3; CPP-32; SCA-1; Yama; PARP Cleavage Protease; Procaspase3; EC 3.4.22
Background

Gene Name: CASP3

NCBI Gene Entry: 836

UniProt Entry: P42574

Application Information

Molecular Weight: Predicted, 32 kDa; observed, 26 kDa

Clonality: Rabbit polyclonal antibody

Species Reactivity: Human, rat

Applications Tested: Western blotting (WB)

Immunogen A synthesized peptide derived from human Caspase 3
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:500-1:2,500
Note This product is for research use only.
Picture
  • Western blotting analysis using anti-caspase 3 antibody (Cat#64941). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-caspase 3 antibody (Cat#64941, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using anti-caspase 3 antibody (Cat #64941). Caspase 3 expression in wild-type (WT) and caspase 3 (CASP3) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-caspase 3 antibody (Cat #64941, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat #201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat #716).
Citation(0)
Western blotting analysis using anti-caspase 3 antibody (Cat#64941). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-caspase 3 antibody (Cat#64941, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-caspase 3 antibody (Cat #64941). Caspase 3 expression in wild-type (WT) and caspase 3 (CASP3) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-caspase 3 antibody (Cat #64941, 1:2,500) and HRP-conjugated goat anti-rabbit secondary antibody (Cat #201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat #716).
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