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KD-Validated Anti-Septin 7 Rabbit Monoclonal Antibody #65602

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Western blotting analysis using anti-septin 7 antibody (Cat#65602). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-septin 7 antibody (Cat#65602, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-septin 7 antibody (Cat#65602). Septin 7 expression in wild-type (WT) and septin 7 (SEPTIN7) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-septin 7 antibody (Cat#65602, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Validation of Septin 7 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-Septin 7 antibody (Cat#65602, 1:2,000) and analyzed using BD flow cytometer.
  • 基因名:
    SEPTIN7
  • 货号:
    65602
  • 应用:
    WB, FCM
  • 物种反应性:
    H, M, R
规格:
20 μL,50 μL,100 μL
价格:
¥ 650.00,¥ 1,350.00,¥ 2,500.00
Information
Product Name KD-Validated Anti-Septin 7 Rabbit Monoclonal Antibody
Aliases SEPTIN7; Septin 7; Septin-7; SEPT7A; CDC10; SEPT7; CDC3; CDC10 Protein Homolog; CDC10 (Cell Division Cycle 10, S. Cerevisiae, Homolog); CDC10 Cell Division Cycle 10 Homolog (S. Cerevisiae); NBLA02942
Background

Gene Name: SEPTIN7

NCBI Gene Entry: 989

UniProt Entry: Q16181

Application Information

Molecular Weight: Predicted, 51 kDa; observed, 45 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 25GB4470

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM)

Immunogen Recombinant protein of human Septin 7
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Note This product is for research use only.
Picture
  • Western blotting analysis using anti-septin 7 antibody (Cat#65602). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-septin 7 antibody (Cat#65602, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Western blotting analysis using anti-septin 7 antibody (Cat#65602). Septin 7 expression in wild-type (WT) and septin 7 (SEPTIN7) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-septin 7 antibody (Cat#65602, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
  • Validation of Septin 7 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-Septin 7 antibody (Cat#65602, 1:2,000) and analyzed using BD flow cytometer.
Citation(0)
Western blotting analysis using anti-septin 7 antibody (Cat#65602). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-septin 7 antibody (Cat#65602, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-septin 7 antibody (Cat#65602). Septin 7 expression in wild-type (WT) and septin 7 (SEPTIN7) shRNA knockdown (KD) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-septin 7 antibody (Cat#65602, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Validation of Septin 7 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-Septin 7 antibody (Cat#65602, 1:2,000) and analyzed using BD flow cytometer.
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