Flow cytometric analysis of Integrin alpha 5 expression in C2C12 cells using Integrin alpha 5 antibody (Cat#67315, 1:2,000). Green, isotype control; red, Integrin alpha 5.
Immunocytochemical staining of C2C12 cells with Integrin alpha 5 antibody (Cat#67315, 1:1,000). Nuclei were stained blue with DAPI; Integrin alpha 5 was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: High. Scale bar: 20 μm.
Western blotting analysis using anti-Integrin alpha 5 antibody (Cat#67315). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Integrin alpha 5 antibody (Cat#67315, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:50,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-Integrin alpha 5 antibody (Cat#67315). Integrin alpha 5 expression in wild type (WT) and Integrin alpha 5 shRNA knockdown (KD) HeLa cells with 30 μg of total cell lysates. β-Tubulin serves as a loading control. The blot was incubated with anti-Integrin alpha 5 antibody (Cat#67315, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Validation of Integrin alpha 5 knockdown using flow cytometry. Wild-type(WT, Blue) and knockdown(KD, Green) HeLa cells were stained with anti-Integrin alpha 5 antibody (Cat#67315, 1:2,000) and analyzed using CytoFLEX.
