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KD-Validated Anti-Phospho-AKT1 (S129) Rabbit Monoclonal Antibody #67471

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Flow cytometric analysis of Phospho-AKT1 (S129) expression in C2C12 cells using Phospho-AKT1 (S129) antibody (Cat#67471, 1:2,000). Green, isotype control; red, Phospho-AKT1 (S129).
Immunocytochemical staining of C2C12 cells with anti-Phospho-AKT1 (S129) antibody (Cat#67471, 1:1,000) . Nuclei were stained blue with DAPI; Phospho-AKT1 (S129) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-Phospho-AKT1 (S129) antibody (Cat#67471). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Phospho-AKT1 (S129) antibody (Cat#67471, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-Phospho-AKT1 (S129) antibody (Cat#67471).Phospho-AKT1 (S129) expression in wild type (WT) and phospho-AKT1 (S129) shRNA knockdown (KD) Hela cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Phospho-AKT1 (S129) antibody (Cat#67471, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名：
    AKT1
  • 货号：
    67471
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00

Information

Picture

Citation(0)

Information
Product Name KD-Validated Anti-Phospho-AKT1 (S129) Rabbit Monoclonal Antibody
Aliases AKT1; AKT Serine/Threonine Kinase 1; RAC; PKB; PRKBA; AKT; V-Akt Murine Thymoma Viral Oncogene Homolog 1; RAC-Alpha Serine/Threonine-Protein Kinase; Protein Kinase B Alpha; Proto-Oncogene C-Akt; Protein Kinase B; RAC-PK-Alpha; EC 2.7.11.1; RAC-ALPHA; PKB Alpha; V-Akt Murine Thymoma Viral Oncogene-Like Protein 1; Serine-Threonine Protein Kinase; Rac Protein Kinase Alpha; RAC-Alpha; PKB-ALPHA; EC 2.7.11; AKT1m
Background

Gene Name: AKT1

NCBI Gene Entry: 207

UniProt Entry: P31749
Application Information

Molecular Weight: Predicted, 56 kDa, observed, 60 kDa

Clonality: Rabbit monoclonal antibody

Clone ID: 23GB1785

Species Reactivity: Human, mouse, rat

Applications Tested: Western blotting (WB), flow cytometry (FCM), immunocytochemistry (IC)
Immunogen A synthesized peptide derived from human AKT1
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:1,000-1:5,000
Flow Cytometry (FCM): 1:2,000
Immunocytochemistry (IC): 1:100-1:1,000
Note This product is for research use only.
Picture
  • Flow cytometric analysis of Phospho-AKT1 (S129) expression in C2C12 cells using Phospho-AKT1 (S129) antibody (Cat#67471, 1:2,000). Green, isotype control; red, Phospho-AKT1 (S129).
  • Immunocytochemical staining of C2C12 cells with anti-Phospho-AKT1 (S129) antibody (Cat#67471, 1:1,000) . Nuclei were stained blue with DAPI; Phospho-AKT1 (S129) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
  • Western blotting analysis using anti-Phospho-AKT1 (S129) antibody (Cat#67471). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Phospho-AKT1 (S129) antibody (Cat#67471, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • Western blotting analysis using anti-Phospho-AKT1 (S129) antibody (Cat#67471).Phospho-AKT1 (S129) expression in wild type (WT) and phospho-AKT1 (S129) shRNA knockdown (KD) Hela cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Phospho-AKT1 (S129) antibody (Cat#67471, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
  • 基因名：
    AKT1
  • 货号：
    67471
  • 应用：
    WB, FCM, IC
  • 物种反应性：
    H, M, R
规格：
20 μL，50 μL，100 μL
价格：
¥ 650.00，¥ 1,350.00，¥ 2,500.00
RELATED PRODUCTS
Flow cytometric analysis of Phospho-AKT1 (S129) expression in C2C12 cells using Phospho-AKT1 (S129) antibody (Cat#67471, 1:2,000). Green, isotype control; red, Phospho-AKT1 (S129).
Immunocytochemical staining of C2C12 cells with anti-Phospho-AKT1 (S129) antibody (Cat#67471, 1:1,000) . Nuclei were stained blue with DAPI; Phospho-AKT1 (S129) was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-Phospho-AKT1 (S129) antibody (Cat#67471). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-Phospho-AKT1 (S129) antibody (Cat#67471, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-Phospho-AKT1 (S129) antibody (Cat#67471).Phospho-AKT1 (S129) expression in wild type (WT) and phospho-AKT1 (S129) shRNA knockdown (KD) Hela cells with 30 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-Phospho-AKT1 (S129) antibody (Cat#67471, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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