Flow cytometric analysis of Cystathionine gamma-lyase expression in HAP-1 cells using Cystathionine gamma-lyase antibody (Cat#71186, 1:2,000). Green, isotype control; red, Cystathionine gamma-lyase.
Immunocytochemical staining of HAP-1 cells with anti-Cystathionine gamma-lyase antibody (Cat#71186, 1:1,000). Nuclei were stained blue with DAPI; Cystathionine gamma-lyase was stained magenta with Alexa Fluor® 647. Images were taken using Leica stellaris 5. Protein abundance based on laser Intensity and smart gain: Medium. Scale bar, 20 μm.
Western blotting analysis using anti-cystathionine gamma-lyase antibody (Cat#71186). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-cystathionine gamma-lyase antibody (Cat#71186, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Western blotting analysis using anti-cystathionine gamma-lyase antibody (Cat#71186). Cystathionine gamma-lyase expression in wild-type (WT) and cystathionine gamma-lyase (CTH) knockout (KO) 293T cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-cystathionine gamma-lyase antibody (Cat#71186, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
