Flow cytometric analysis of Zinc finger protein 217 expression in HAP-1 cells using anti-Zinc finger protein 217 antibody (Cat#71230, 1:2,000). Green, isotype control; red, Zinc finger protein 217.
Western blotting analysis using anti-zinc finger protein 217 antibody (Cat#71230). Total cell lysates (30 μg) from various cell lines were loaded and separated by SDS-PAGE. The blot was incubated with anti-zinc finger protein 217 antibody (Cat#71230, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).
Western blotting analysis using anti-zinc finger protein 217 antibody (Cat#71230). Zinc finger protein 217 expression in wild-type (WT) and zinc finger protein 217 (ZNF217) knockout (KO) HeLa cells with 20 μg of total cell lysates. Hsp90 α serves as a loading control. The blot was incubated with anti-zinc finger protein 217 antibody (Cat#71230, 1:5,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using NaQ™ ECL Substrate Kit (Cat#716).