Product Name: NaQ™ ECL Substrate Kit

Background:

Enhanced chemiluminescence (ECL) reagents are used with horseradish peroxidase (HRP)-conjugated secondary antibodies to detect protein abundance in Western blot. HRP catalyzes luminol oxidation in the presence of hydrogen peroxide, producing light that can be detected by X-ray film and digital imagers. Our NaQ™ reagents are formulated with proprietary enhancers that greatly increase the intensity and duration of emitted light. Our NaQ™ ECL reagents are suitable to detect proteins within the high picogram to low nanogram range. When using NaQ™ ECL reagents, the recommended dilutions for primary and secondary antibodies are 1:1,000-2,000 and 1:10,000-20,000, respectively. Compared to other commonly used ECL reagents, ours exhibit excellent performance by generating intense signal with low background and req

Application Information

Allow the ECL reagents to equilibRate to room tempeRature approximately 30 min before use. Wash the membrane thoroughly to reduce non-specific signals. Mix Reagent A and B at a 1:1 Ratio. Vortex to mix. Immerse the membrane in the mixed solution for 2–3 min. Drain the excess solution using a paper towel and wrap the membrane in plastic wrap or a clear plastic folder. Detect the chemiluminescence using X-ray film or a digital imager.

Storage

Stable at 4°C for at least 1 year.