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Human HSPA8 Knockdown Cell Line (Wb-Validated) #C61475

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RT-qPCR analysis. 293T cells were infected with HSPA8-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.

Western blotting analysis. HSPA8 protein expression in wild-type (WT) and shRNA knockdown (KD) 293T cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies (Cat#61475, 1:5,000) against HSPA8 and β-Tubulin , respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000). Images were developed using FeQ™ ECL Substrate Kit (Cat#226).

基因名：

HSPA8
货号：

C61475
应用：
物种反应性：

规格：

1 Kit

价格：

询价

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Product Name	Human HSPA8 Knockdown Cell Line (Wb-Validated)
Aliases	HSPA8; Heat Shock Protein Family A (Hsp70) Member 8; HSC70; HSP73; HSPA10; HSC71; Lipopolysaccharide-Associated Protein 1; Heat Shock Cognate 71 KDa Protein; Heat Shock 70kDa Protein 8; LPS-Associated Protein 1; LAP-1; Epididymis Secretory Sperm Binding Protein Li 72p; N-Myristoyltransferase Inhibitor Protein 71; Constitutive Heat Shock Protein 70; Epididymis Luminal Protein 33; Heat Shock Cognate Protein 54; Heat Shock 70 KDa Protein 8; Heat Shock 70kd Protein 10; Heat Shock 70kD Protein 8; EC 3.6.4.10; HEL-S-72p; HEL-33; HSC54; HSP71; NIP71; LAP1
Background	Gene Name: HSPA8 NCBI Gene Entry: 3312
Storage	Store at liquid nitrogen for 1 year.
Kit Components	1. Human HSPA8 Knockdown Cell Line (Wb-Validated) 2. Wild-type cell line
Parental Cell Line	Human cell line supplied by the client
Validation Methods	RT-qPCR, Western blotting (WB)
Shipping	Shipped on Dry Ice.
Instructions For Use	This knockdown cell line should be paired with wild-type cell line for use.
Note	This product is for research use only.

Picture

RT-qPCR analysis. 293T cells were infected with HSPA8-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. HSPA8 protein expression in wild-type (WT) and shRNA knockdown (KD) 293T cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies (Cat#61475, 1:5,000) against HSPA8 and β-Tubulin , respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000). Images were developed using FeQ™ ECL Substrate Kit (Cat#226).

Citation(0)

基因名：

HSPA8
货号：

C61475
应用：
物种反应性：

规格：

1 Kit

价格：

询价

Datasheet

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