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Human NUDT9 Knockdown Cell Line (Wb-Validated) #C65587

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RT-qPCR analysis. HeLa cells were infected with NUDT9-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis.NUDT9 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting.  Hsp90 α served as a loading control. The blots were incubated with primary antibodies against NUDT9 and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using NaQ™ ECL Substrate Kit.
  • 基因名:
    NUDT9
  • 货号:
    C65587
  • 应用:
  • 物种反应性:
规格:
1 Kit
价格:
询价
Information
Product Name Human NUDT9 Knockdown Cell Line (Wb-Validated)
Aliases NUDT9; Nudix Hydrolase 9; Nudix (Nucleoside Diphosphate Linked Moiety X)-Type Motif 9; ADP-Ribose Pyrophosphatase, Mitochondrial; Adenosine Diphosphoribose Pyrophosphatase; ADP-Ribose Phosphohydrolase; ADP-Ribose Diphosphatase; ADPR-PPase; MGC3037; NUDT10; Nucleoside Diphosphate Linked Moiety X-Type Motif 9; Nucleoside Diphosphate-Linked Moiety X Motif 9; ADP-Ribose Pyrosphosphatase NUDT9; Nudix Motif 9; EC 3.6.1.13
Background

Gene Name: NUDT9

NCBI Gene Entry: 53343

Storage Store at liquid nitrogen for 1 year.
Kit Components 1. Human NUDT9 Knockdown Cell Line (Wb-Validated)
2. Wild-type cell line
Parental Cell Line Human cell line supplied by the client
Validation Methods RT-qPCR, Western blotting (WB)
Shipping Shipped on Dry Ice.
Instructions For Use This knockdown cell line should be paired with wild-type cell line for use.
Note This product is for research use only.
Picture
  • RT-qPCR analysis. HeLa cells were infected with NUDT9-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
  • Western blotting analysis.NUDT9 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting.  Hsp90 α served as a loading control. The blots were incubated with primary antibodies against NUDT9 and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using NaQ™ ECL Substrate Kit.
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RT-qPCR analysis. HeLa cells were infected with NUDT9-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis.NUDT9 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting.  Hsp90 α served as a loading control. The blots were incubated with primary antibodies against NUDT9 and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using NaQ™ ECL Substrate Kit.
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