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Human ARHGAP5 Knockdown Cell Line (Wb-Validated) #C61634

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RT-qPCR analysis. HeLa cells were infected with ARHGAP5-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. ARHGAP5 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies (Cat#61634, 1:5,000) against ARHGAP5 and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000). Images were developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名:
    ARHGAP5
  • 货号:
    C61634
  • 应用:
  • 物种反应性:
规格:
1 Kit
价格:
询价
Information
Product Name Human ARHGAP5 Knockdown Cell Line (Wb-Validated)
Aliases ARHGAP5; Rho GTPase Activating Protein 5; P190-B; Rho-Type GTPase-Activating Protein 5; Rho GTPase-Activating Protein 5; Growth Factor Independent 2; P190BRhoGAP; RhoGAP5; RHOGAP5; GFI2; P100 RasGAP-Associated P105 Protein; P105 RhoGAP; P190BRHOGAP
Background

Gene Name: ARHGAP5

NCBI Gene Entry: 394

Storage Store at liquid nitrogen for 1 year.
Kit Components 1. Human ARHGAP5 Knockdown Cell Line (Wb-Validated)
2. Wild-type cell line
Parental Cell Line Human cell line supplied by the client
Validation Methods RT-qPCR, Western blotting (WB)
Shipping Shipped on Dry Ice.
Instructions For Use This knockdown cell line should be paired with wild-type cell line for use.
Note This product is for research use only.
Picture
  • RT-qPCR analysis. HeLa cells were infected with ARHGAP5-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
  • Western blotting analysis. ARHGAP5 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies (Cat#61634, 1:5,000) against ARHGAP5 and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000). Images were developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
RT-qPCR analysis. HeLa cells were infected with ARHGAP5-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. ARHGAP5 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies (Cat#61634, 1:5,000) against ARHGAP5 and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000). Images were developed using FeQ™ ECL Substrate Kit (Cat#226).
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