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Human KIF2C Knockdown Cell Line (Wb-Validated) #C63295

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RT-qPCR analysis. 293T cells were infected with KIF11-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.

Western blotting analysis. KIF2C protein expression in wild-type (WT) and shRNA knockdown (KD) 293T cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against KIF2C and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.

基因名：

KIF2C
货号：

C63295
应用：
物种反应性：

规格：

1 Kit

价格：

询价

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Product Name	Human KIF2C Knockdown Cell Line (Wb-Validated)
Aliases	KIF2C; Kinesin Family Member 2C; MCAK; CT139; KNSL6; Mitotic Centromere-Associated Kinesin; Kinesin-Like Protein KIF2C; Kinesin-Like Protein 6; Kinesin-Like 6 (Mitotic Centromere-Associated Kinesin); Testis Tissue Sperm-Binding Protein Li 68n
Background	Gene Name: KIF2C NCBI Gene Entry: 11004
Storage	Store at liquid nitrogen for 1 year.
Kit Components	1. Human KIF2C Knockdown Cell Line (Wb-Validated) 2. Wild-type cell line
Parental Cell Line	Human cell line supplied by the client
Validation Methods	RT-qPCR, Western blotting (WB)
Shipping	Shipped on Dry Ice.
Instructions For Use	This knockdown cell line should be paired with wild-type cell line for use.
Note	This product is for research use only.

Picture

RT-qPCR analysis. 293T cells were infected with KIF11-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. KIF2C protein expression in wild-type (WT) and shRNA knockdown (KD) 293T cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against KIF2C and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.

Citation(0)

基因名：

KIF2C
货号：

C63295
应用：
物种反应性：

规格：

1 Kit

价格：

询价

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