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Human DEGS1 Knockdown Cell Line (Wb-Validated) #C62231

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RT-qPCR analysis. HT-1080 cells were infected with DEGS1-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. DEGS1 protein expression in wild-type (WT) and shRNA knockdown (KD) HT-1080 cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies against DEGS1 and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
  • 基因名：
    DEGS1
  • 货号：
    C62231
  • 应用：
  • 物种反应性：
规格：
1 Kit
价格：
询价

Information

Picture

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Information
Product Name Human DEGS1 Knockdown Cell Line (Wb-Validated)
Aliases DEGS1; Delta 4-Desaturase, Sphingolipid 1; DES1; MLD; DEGS-1; Des-1; FADS7; Cell Migration-Inducing Gene 15 Protein; Sphingolipid Delta(4)-Desaturase DES1; Sphingolipid Delta(4)-Desaturase 1; Dihydroceramide Desaturase 1; Membrane Lipid Desaturase; Retinol Isomerase; Degenerative Spermatocyte Homolog 1, Lipid Desaturase (Drosophila); Degenerative Spermatocyte Homolog 1, Lipid Desaturase; Degenerative Spermatocyte Homolog, Lipid Desaturase; Membrane Fatty Acid (Lipid) Desaturase; Degenerative Spermatocyte Homolog 1; Migration-Inducing Gene 15 Protein; Sphingolipid Delta 4 Desaturase; Dihydroceramide Desaturase-1; EC 1.14.19.17; EC 5.2.1.-; HLD18; MIG15; DEGS
Background

Gene Name: DEGS1

NCBI Gene Entry: 8560
Storage Store at liquid nitrogen for 1 year.
Kit Components 1. Human DEGS1 Knockdown Cell Line (Wb-Validated)
2. Wild-type cell line
Parental Cell Line Human cell line supplied by the client
Validation Methods RT-qPCR, Western blotting (WB)
Shipping Shipped on Dry Ice.
Instructions For Use This knockdown cell line should be paired with wild-type cell line for use.
Note This product is for research use only.
Picture
  • RT-qPCR analysis. HT-1080 cells were infected with DEGS1-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
  • Western blotting analysis. DEGS1 protein expression in wild-type (WT) and shRNA knockdown (KD) HT-1080 cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies against DEGS1 and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
Citation(0)
  • 基因名：
    DEGS1
  • 货号：
    C62231
  • 应用：
  • 物种反应性：
规格：
1 Kit
价格：
询价
RELATED PRODUCTS
RT-qPCR analysis. HT-1080 cells were infected with DEGS1-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. DEGS1 protein expression in wild-type (WT) and shRNA knockdown (KD) HT-1080 cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies against DEGS1 and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
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