首页

关于我们

善本标准

产品中心

定制服务

信号通路

促销活动

技术支持

新闻资讯

  • 一抗
  • 二抗
  • 慢病毒
  • 细胞系
  • 裂解物
  • 试剂类
首页>产品中心>细胞系

Human SH3GL1 Knockdown Cell Line (Wb-Validated) #C65029

一键复制产品信息

返回列表

RT-qPCR analysis. HeLa cells were infected with SH3GL1-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. SH3GL1 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against SH3GL1 and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-mouse secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
  • 基因名：
    SH3GL1
  • 货号：
    C65029
  • 应用：
  • 物种反应性：
规格：
1 Kit
价格：
询价

Information

Picture

Citation(0)

Information
Product Name Human SH3GL1 Knockdown Cell Line (Wb-Validated)
Aliases SH3GL1; SH3 Domain Containing GRB2 Like 1, Endophilin A2; SH3D2B; CNSA1; EEN; SH3P8; Extra Eleven-Nineteen Leukemia Fusion Gene Protein; SH3 Domain-Containing GRB2-Like Protein 1; SH3-Containing Grb-2-Like 1 Protein; Extra 11-19 Leukemia Fusion; EEN Fusion Partner Of MLL; SH3-Domain GRB2-Like 1; SH3 Domain Protein 2B; Endophilin-A2; Endophilin-2; MGC111371; SH3 Domain Containing GRB2 Like 1; SH3-Containing Protein EEN; SH3 Domain GRB2-Like 1; Fusion Partner Of MLL; Endophilin A2
Background

Gene Name: SH3GL1

NCBI Gene Entry: 6455
Storage Store at liquid nitrogen for 1 year.
Kit Components 1. Human SH3GL1 Knockdown Cell Line (Wb-Validated)
2. Wild-type cell line
Parental Cell Line Human cell line supplied by the client
Validation Methods RT-qPCR, Western blotting (WB)
Shipping Shipped on Dry Ice.
Instructions For Use This knockdown cell line should be paired with wild-type cell line for use.
Note This product is for research use only.
Picture
  • RT-qPCR analysis. HeLa cells were infected with SH3GL1-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
  • Western blotting analysis. SH3GL1 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against SH3GL1 and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-mouse secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
Citation(0)
  • 基因名：
    SH3GL1
  • 货号：
    C65029
  • 应用：
  • 物种反应性：
规格：
1 Kit
价格：
询价
RELATED PRODUCTS
RT-qPCR analysis. HeLa cells were infected with SH3GL1-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. SH3GL1 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against SH3GL1 and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-mouse secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
  • 联系电话

    0551-63635518

    0551-65578998

  • 联系邮箱

    support@genuinbiotech.cn

    sales@genuinbiotech.cn

微信公众号

Copyright © 2025 合肥善本生物科技有限公司

All Rights Reserved. 皖ICP备2023000814号