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Human RPS6KB2 Knockdown Cell Line (Wb-Validated) #C62521

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RT-qPCR analysis. HeLa cells were infected with RPS6KB2-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. RPS6KB2 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against RPS6KB2 and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
  • 基因名：
    RPS6KB2
  • 货号：
    C62521
  • 应用：
  • 物种反应性：
规格：
1 Kit
价格：
询价

Information

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Information
Product Name Human RPS6KB2 Knockdown Cell Line (Wb-Validated)
Aliases RPS6KB2; Ribosomal Protein S6 Kinase B2; STK14B; P70-BETA; P70S6Kb; S6Kbeta; S6KB; KLS; 70 KDa Ribosomal Protein S6 Kinase 2; Serine/Threonine-Protein Kinase 14B; Ribosomal Protein S6 Kinase Beta-2; P70 Ribosomal S6 Kinase Beta; S6 Kinase-Related Kinase; P70 S6 Kinase Beta; P70 S6K-Beta; EC 2.7.11.1; S6K-Beta-2; P70-S6K 2; S6K-Beta; P70 S6KB; P70S6K2; S6K2; SRK; Ribosomal Protein S6 Kinase, 70kDa, Polypeptide 2; Ribosomal Protein S6 Kinase, 70kD, Polypeptide 2; Serine/Threonine-Protein Kinase 14 Beta; P70(S6K)-Beta; P70-Beta-1; P70-Beta-2; S6K-Beta2; EC 2.7.11; P70-Beta; S6KI(2); S6Kβ
Background

Gene Name: RPS6KB2

NCBI Gene Entry: 6199
Storage Store at liquid nitrogen for 1 year.
Kit Components 1. Human RPS6KB2 Knockdown Cell Line (Wb-Validated)
2. Wild-type cell line
Parental Cell Line Human cell line supplied by the client
Validation Methods RT-qPCR, Western blotting (WB)
Shipping Shipped on Dry Ice.
Instructions For Use This knockdown cell line should be paired with wild-type cell line for use.
Note This product is for research use only.
Picture
  • RT-qPCR analysis. HeLa cells were infected with RPS6KB2-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
  • Western blotting analysis. RPS6KB2 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against RPS6KB2 and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
Citation(0)
  • 基因名：
    RPS6KB2
  • 货号：
    C62521
  • 应用：
  • 物种反应性：
规格：
1 Kit
价格：
询价
RELATED PRODUCTS
RT-qPCR analysis. HeLa cells were infected with RPS6KB2-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. RPS6KB2 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against RPS6KB2 and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
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