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Human TAF15 Knockdown Cell Line (Wb-Validated) #C62622

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RT-qPCR analysis. HeLa cells were infected with TAF15-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.

Western blotting analysis. TAF15 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies against TAF15 and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.

基因名：

TAF15
货号：

C62622
应用：
物种反应性：

规格：

1 Kit

价格：

询价

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Product Name	Human TAF15 Knockdown Cell Line (Wb-Validated)
Aliases	TAF15; TATA-Box Binding Protein Associated Factor 15; RBP56; TAF2N; Npl3; TATA Box Binding Protein (TBP)-Associated Factor, RNA Polymerase II, N, 68kD (RNA-Binding Protein 56); TAF15 RNA Polymerase II, TATA Box Binding Protein (TBP)-Associated Factor, 68kDa; TATA-Binding Protein-Associated Factor 2N; HTAFII68; TAFII68; TATA Box-Binding Protein-Associated Factor 2N (RNA-Binding Protein 56); 68 KDa TATA-Binding Protein-Associated Factor; TBP-Associated Factor 15; RNA-Binding Protein 56; RBP56/CSMF Fusion; TAF(II)68
Background	Gene Name: TAF15 NCBI Gene Entry: 8148
Storage	Store at liquid nitrogen for 1 year.
Kit Components	1. Human TAF15 Knockdown Cell Line (Wb-Validated) 2. Wild-type cell line
Parental Cell Line	Human cell line supplied by the client
Validation Methods	RT-qPCR, Western blotting (WB)
Shipping	Shipped on Dry Ice.
Instructions For Use	This knockdown cell line should be paired with wild-type cell line for use.
Note	This product is for research use only.

Picture

RT-qPCR analysis. HeLa cells were infected with TAF15-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. TAF15 protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. β-Tubulin served as a loading control. The blots were incubated with primary antibodies against TAF15 and β-Tubulin, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.

Citation(0)

基因名：

TAF15
货号：

C62622
应用：
物种反应性：

规格：

1 Kit

价格：

询价

Datasheet

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