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Human EIF3B Knockdown Cell Line (Wb-Validated) #C63215

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RT-qPCR analysis. HeLa cells were infected with EIF3B-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. EIF3B protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against EIF3B and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
  • 基因名:
    EIF3B
  • 货号:
    C63215
  • 应用:
  • 物种反应性:
规格:
1 Kit
价格:
询价
Information
Product Name Human EIF3B Knockdown Cell Line (Wb-Validated)
Aliases EIF3B; Eukaryotic Translation Initiation Factor 3 Subunit B; EIF3S9; PRT1; Eukaryotic Translation Initiation Factor 3, Subunit 9 Eta, 116kDa; Eukaryotic Translation Initiation Factor 3 Subunit 9; Protein Synthesis 1; Prt1 Homolog; Eukaryotic Translation Initiation Factor 3, Subunit 9 (Eta, 116kD); Eukaryotic Translation Initiation Factor 3, Subunit B; EIF3-P110; EIF3-P116; EIF-3-Eta; EIF3 P110; EIF3 P116; EIF3-ETA; EIF3b; HPrt1
Background

Gene Name: EIF3B

NCBI Gene Entry: 8662

Storage Store at liquid nitrogen for 1 year.
Kit Components 1. Human EIF3B Knockdown Cell Line (Wb-Validated)
2. Wild-type cell line
Parental Cell Line Human cell line supplied by the client
Validation Methods RT-qPCR, Western blotting (WB)
Shipping Shipped on Dry Ice.
Instructions For Use This knockdown cell line should be paired with wild-type cell line for use.
Note This product is for research use only.
Picture
  • RT-qPCR analysis. HeLa cells were infected with EIF3B-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
  • Western blotting analysis. EIF3B protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against EIF3B and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
Citation(0)
RT-qPCR analysis. HeLa cells were infected with EIF3B-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. EIF3B protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against EIF3B and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
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