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Human ANXA6 Knockdown Cell Line (Wb-Validated) #C63668

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RT-qPCR analysis. HT-1080 cells were infected with ANXA6-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. ANXA6 protein expression in wild-type (WT) and shRNA knockdown (KD) HT-1080 cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against ANXA6 and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
  • 基因名：
    ANXA6
  • 货号：
    C63668
  • 应用：
  • 物种反应性：
规格：
1 Kit
价格：
询价

Information

Picture

Citation(0)

Information
Product Name Human ANXA6 Knockdown Cell Line (Wb-Validated)
Aliases ANXA6; Annexin A6; ANX6; 67 KDa Calelectrin; Chromobindin-20; Lipocortin VI; Annexin-6; CPB-II; P68; P70; Testis Secretory Sperm-Binding Protein Li 198a; Calcium-Binding Protein P68; Annexin VI (P68); Calphobindin II; Calphobindin-II; Calelectrin; Protein III; Annexin VI; CBP68
Background

Gene Name: ANXA6

NCBI Gene Entry: 309
Storage Store at liquid nitrogen for 1 year.
Kit Components 1. Human ANXA6 Knockdown Cell Line (Wb-Validated)
2. Wild-type cell line
Parental Cell Line Human cell line supplied by the client
Validation Methods RT-qPCR, Western blotting (WB)
Shipping Shipped on Dry Ice.
Instructions For Use This knockdown cell line should be paired with wild-type cell line for use.
Note This product is for research use only.
Picture
  • RT-qPCR analysis. HT-1080 cells were infected with ANXA6-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
  • Western blotting analysis. ANXA6 protein expression in wild-type (WT) and shRNA knockdown (KD) HT-1080 cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against ANXA6 and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
Citation(0)
  • 基因名：
    ANXA6
  • 货号：
    C63668
  • 应用：
  • 物种反应性：
规格：
1 Kit
价格：
询价
RELATED PRODUCTS
RT-qPCR analysis. HT-1080 cells were infected with ANXA6-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. ANXA6 protein expression in wild-type (WT) and shRNA knockdown (KD) HT-1080 cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against ANXA6 and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-rabbit secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
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