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Human DDOST Knockdown Cell Line (Wb-Validated) #C63861

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RT-qPCR analysis. HeLa cells were infected with DDOST-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. DDOST protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against DDOST and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-mouse secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
  • 基因名：
    DDOST
  • 货号：
    C63861
  • 应用：
  • 物种反应性：
规格：
1 Kit
价格：
询价

Information

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Information
Product Name Human DDOST Knockdown Cell Line (Wb-Validated)
Aliases DDOST; Dolichyl-Diphosphooligosaccharide--Protein Glycosyltransferase Non-Catalytic Subunit; OST48; KIAA0115; WBP1; OST; Dolichyl-Diphosphooligosaccharide--Protein Glycosyltransferase 48 KDa Subunit; Advanced Glycation End-Product Receptor 1; Oligosaccharyl Transferase 48 KDa Subunit; Oligosaccharyltransferase Subunit 48; Dolichyl-Diphosphooligosaccharide--Protein Glycosyltransferase Subunit (Non-Catalytic); Dolichyl-Diphosphooligosaccharide-Protein Glycosyltransferase; Dolichyl-Diphosphooligosaccharide-Protein Glycotransferase; Advanced Glycation Endproduct Receptor 1; Oligosaccharyltransferase 48 KDa Subunit; DDOST 48 KDa Subunit; EC 2.4.1.119; OKSWcl45; AGER1; CDG1R; GATD6
Background

Gene Name: DDOST

NCBI Gene Entry: 1650
Storage Store at liquid nitrogen for 1 year.
Kit Components 1. Human DDOST Knockdown Cell Line (Wb-Validated)
2. Wild-type cell line
Parental Cell Line Human cell line supplied by the client
Validation Methods RT-qPCR, Western blotting (WB)
Shipping Shipped on Dry Ice.
Instructions For Use This knockdown cell line should be paired with wild-type cell line for use.
Note This product is for research use only.
Picture
  • RT-qPCR analysis. HeLa cells were infected with DDOST-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
  • Western blotting analysis. DDOST protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against DDOST and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-mouse secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
Citation(0)
  • 基因名：
    DDOST
  • 货号：
    C63861
  • 应用：
  • 物种反应性：
规格：
1 Kit
价格：
询价
RELATED PRODUCTS
RT-qPCR analysis. HeLa cells were infected with DDOST-specific shRNA lentiviral particles, total RNA was extracted from wild-type and knockdown cells, RT-qPCR was performed using gene-specific primers. ∆Ct (CtKD-CtWT) was used to calculate mRNA reduction (%) between wild-type and knockdown cells using the following formula: (1-1/2∆Ct) x 100%.
Western blotting analysis. DDOST protein expression in wild-type (WT) and shRNA knockdown (KD) HeLa cells was detected using Western blotting. Hsp90 α served as a loading control. The blots were incubated with primary antibodies against DDOST and Hsp90 α, respectively, followed by incubating with HRP-conjugated goat anti-mouse secondary antibody. Images were developed using FeQ™ ECL Substrate Kit.
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