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Anti-V5 Tag Rabbit Monoclonal Antibody #T003

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Western blotting analysis using anti-V5-tag antibody (Cat#T003). Recombinant multi-tag protein (5 ng) in E. coli were loaded and separated by SDS-PAGE. The blot was incubated with anti-V5-tag antibody (Cat#T003, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
  • 基因名:
  • 货号:
    T003
  • 应用:
    WB
  • 物种反应性:
    Recombinant protein
规格:
20 μL,50 μL,100 μL
价格:
¥ 310.00,¥ 600.00,¥ 1,000.00
Information
Product Name Anti-V5 Tag Rabbit Monoclonal Antibody
Aliases V5-tag; GKPIPNPLLGLDST epitope tag; GKPIPNPLLGLDST tag; Protein Rev; Regulator of expression of viral proteins; rev; V5 epitope tag
Background The V5-tag is a short, artificial peptide sequence (GKPIPNPLLGLDST) derived from a small epitope present in the P and V proteins of the paramyxovirus simian virus 5 (SV5). It is genetically engineered and fused to either the N-terminus or C-terminus of a recombinant protein of interest, primarily serving as an epitope tag. Unlike affinity tags like the His-tag, its main purpose is not purification but rather highly specific detection and localization.
Application Information

Molecular Weight: Recombinant protein dependent

Clonality: Mouse monoclonal antibody

Species Reactivity: Recombinant protein

Applications Tested: Western blotting (WB)

Immunogen Recombinant Protein of V5 tag
Isotype Rabbit IgG
Storage Buffer Supplied in PBS (pH 7.4) containing 50% glycerol, and 0.02% sodium azide.
Storage Store at -20 °C for one year.
Recommended Dilutions Western Blotting (WB): 1:2,000-1:10,000
Note This product is for research use only.
Picture
  • Western blotting analysis using anti-V5-tag antibody (Cat#T003). Recombinant multi-tag protein (5 ng) in E. coli were loaded and separated by SDS-PAGE. The blot was incubated with anti-V5-tag antibody (Cat#T003, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
Citation(0)
Western blotting analysis using anti-V5-tag antibody (Cat#T003). Recombinant multi-tag protein (5 ng) in E. coli were loaded and separated by SDS-PAGE. The blot was incubated with anti-V5-tag antibody (Cat#T003, 1:10,000) and HRP-conjugated goat anti-rabbit secondary antibody (Cat#201, 1:20,000) respectively. Image was developed using FeQ™ ECL Substrate Kit (Cat#226).
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